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  • 1
    Electronic Resource
    Electronic Resource
    Characterization and comparison of merozoite antigens of differentBabesia canis isolates by serological and immunological investigations (1995)
    Springer
    Parasitology research 81 (1995), S. 638-642 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Merozoites of fourBabesia canis isolates from Hungary, France, Africa, and Egypt were purified. Antigens were compared in an enzyme-linked immunosorbent assay (ELISA) and by immunoblotting. In the ELISA, antigen from the highly pathogenic isolate from Hungary showed the highest sensitivity for homologous and heterologous immune sera. This was confirmed by immunoblotting. Protein bands of the Hungarian isolate were strongly recognized by allB. canis immune sera, whereas the antigens from the other isolates showed only weak reactions with homologous and heterologous immune sera. Significant was a protein band of about 12 kDa appearing in all pathogenic isolates from Hungary, France, and South Africa but not in the apathogenic Egyptian isolate. This protein band may determine the virulence. For serological tests, theB. canis isolate from Hungary seems to be the one most suitable for detection of even mild infections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00931839
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Ribosomal small-subunit RNA gene-sequence analysis of Theileria lestoquardi and a Theileria species highly pathogenic for small ruminants in China (2000)
    Springer
    Parasitology research 86 (2000), S. 352-358 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A fatal disease of sheep and goats in the northwestern part of China has been reported to be due to Theileria lestoquardi (syn. T. hirci). However, some characteristics of the causative agent are not in accordance with attributes ascribed to this parasite. We therefore determined the nucleotide sequence of the small-subunit ribosomal RNA (srRNA) gene of T. lestoquardi and the parasite identified in China and compared it with that of other Theileria and Babesia species. In the inferred phylogenetic tree the srRNA sequence of the Chinese parasite was found to be most closely related to T. buffeli and clearly divergent from T. lestoquardi, suggesting that it is an as yet unrecognized Theileria species. Extensive structural similarities were observed between the srRNA sequences of T. lestoquardi and T. annulata, revealing a close phylogenetic relationship between these two Theileria species. On the basis of the srRNA nucleotide sequence, polymerase chain reaction (PCR) primers were designed that specifically amplified genomic DNA of the Chinese Theileria species. These primers may be valuable tools in future epidemiology studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004360050680
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  • 3
    Electronic Resource
    Electronic Resource
    Is interleukin 2 necessary for the autocrine proliferation of Theileria -infected bovine cells? (1999)
    Springer
    Parasitology research 85 (1999), S. 409-412 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Theileria-infected bovine lymphoblastoid cells are induced to proliferate permanently such that the division of the parasites and that of their host cells occur synchronously. The mechanism by which the parasites induce the transformation of their host cells is unknown. We investigated the growth-factor dependency of a number of Theileria-infected cell lines. Supernatants of the majority of the cell lines tested in our study showed no growth-enhancing activity. However, supernatants of a limited number of cell lines occasionally enhanced the growth of parasitized cells that were used as responder cells. Thus, they contained a growth factor whose biological effect was not eliminated by an anti-interleukin-2 (anti-IL-2) antibody. Moreover, neither the proliferation of T. parva-infected cells nor that of T. annulata-infected cells was impaired by this antibody. In contrast, the anti-IL-2 antibody substantially prevented bovine peripheral blood mononuclear cells from undergoing a proliferative response upon stimulation with concanavalin A (Con A). In line with these results we observed that unlike Con A-stimulated lymphocytes, the infected cell lines did not express IL-2 mRNA. Taken together, our results suggest that Theileria-infected cells do not secrete IL-2 and that IL-2 does not play an important role in the autocrine proliferation of the parasitized host cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004360050567
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  • 4
    Electronic Resource
    Electronic Resource
    The proliferation-associated nuclear protein Ki-67 in the bovine system: partial characterisation and its application for determination of the proliferation of Theileria-infected bovine cells (1999)
    Springer
    Parasitology research 85 (1999), S. 613-620 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Theileria annulata-infected bovine cells as well as mitogen-stimulated bovine peripheral blood mononuclear cells (PBMC) express a proliferation-associated nuclear protein equivalent to the human Ki-67 protein. In analogy to the human system, the expression of the bovine Ki-67 protein is restricted to proliferating cells only, since (a) Ki-67 expression paralleled [3H]-thymidine incorporation in concanavalin A (Con A)-stimulated bovine PBMC, (b) Ki-67 was not detectable in quiescent bovine cells, and (c) Ki-67 expression in Theileria-infected cells is related to the presence of the parasites within the cytoplasm of the host cells; upon treatment with the theilericidal drug buparvaquone the parasites are destroyed and the cells cease to proliferate and to express the Ki-67 protein. Western-blot analysis of lysates of proliferating bovine cells revealed that the prototype monoclonal antibody Ki-67 and the new equivalent antibody MIB-1 detected one prominent protein band with an apparent molecular weight of 430 kDa. Two cDNA clones (pUC18.B1.Ki-67 and pUC18.B2.Ki-67) were isolated from a λgt11 cDNA library of T. annulata-infected bovine cells by immunoscreening with the monoclonal antibody MIB-1. Comparison of these cDNA sequences with those of the human Ki-67 protein revealed 60–70% identity. Within the “Ki-67 motif”, identity proved to be 80% at the amino acid level. The remarkable identity between bovine and human Ki-67 proteins suggests that MIB-1 can be used as a marker for cell proliferation in animal research. In this context we could identify proliferating cells in lymph nodes of Theileria-infected animals and, furthermore, we could distinguish between infected and uninfected proliferating cells using MIB-1 and an antiserum against a recombinant parasite protein designated SA288.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004360050605
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  • 5
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    Sensitivity of Ewing's sarcoma to TRAIL-induced apoptosis (2001)
    Springer Nature
    Details
    Publication Date: 2001-05-01
    Print ISSN: 1350-9047
    Electronic ISSN: 1476-5403
    Topics: Biology , Medicine
    Published by Springer Nature
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