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  • 1
    Electronic Resource
    Electronic Resource
    Vapor-liquid equilibrium in the system carbon dioxide + cyclopentane from 275 to 493 K at pressures to 12.2 MPa (1991)
    s.l. : American Chemical Society
    Journal of chemical & engineering data 36 (1991), S. 188-192 
    Details
    ISSN: 1520-5134
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/je00002a014
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  • 2
    Electronic Resource
    Electronic Resource
    Vapor-liquid equilibrium in the system carbon dioxide + 2,2-dimethylpropane from 262 to 424 K at pressures to 8.4 MPa (1990)
    s.l. : American Chemical Society
    Journal of chemical & engineering data 35 (1990), S. 278-283 
    Details
    ISSN: 1520-5134
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/je00061a015
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  • 3
    Electronic Resource
    Electronic Resource
    Combination of the Mahalanobis distance and residual variance pattern recognition techniques for classification of near-infrared reflectance spectra (1990)
    s.l. : American Chemical Society
    Analytical chemistry 62 (1990), S. 465-470 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac00204a009
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of modifier concentration on the fracture behaviour of rubber-modified PMMA (1988)
    Springer
    Journal of materials science 23 (1988), S. 3623-3629 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Izod fracture surfaces of blends of PMMA with various amounts of a rubber modifier were studied using scanning electron microscopy. Attention was focused on modes of crack initiation and propagation and on the role of the modifier in the fracture process. It was found that the impact strength of this class of materials increased monotonically with an increase in modifier concentration, at least up to 40 wt% modifier. Unmodified PMMA was studied to provide a basis for understanding the morphological features on the fracture surfaces of the rubber-modified blends. It was confirmed that PMMA fractures through the formation and rupture of crazes. This phenomenon was also found to occur in blends containing 10 wt% modifier. However, blends with 20 wt% modifier crazed only in the later stages of the fracture process, when the crack speed had exceeded some critical value. No evidence of crazing was found in blends with 30 and 40 wt% modifier loadings, although extensive plastic deformation was observed on the fracture surfaces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00540504
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  • 5
    Electronic Resource
    Electronic Resource
    Cultivation of Methylosinus trichosporium OB3b: III. Production of particulate methane monooxygenase in continuous culture (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 40 (1992), S. 705-712 
    Details
    ISSN: 0006-3592
    Keywords: methanotroph ; particulate methane monooxygenase ; nitrogenase ; continuous culture conditions ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Continous culture experiments with the obligatory methanotroph, Methylosinus trichosporium OB3b, were conducted to study the whole-cell methane monooxygenase (MMO) and nitrogenase activities in a nitrate minimal salts medium under oxygen-limited conditions with methane as the carbone source. The important variables investigated were the feed medium concentrations of copper and nitrate, CO2 addition, the agitation speed, and the dilution rate. M. trichosporium OB3b required quantitative amounts of copper (2.6 × 10-4 g Cu/g dry cell Wt) for the exclusive production of particulate MMo during continous culture growth. When the feed medium nitrate concentration was varied in the range of 5-50 mM, the whole-cell specific pMMO activity exhibited a maximum at 40 mM. The elimination of external CO2 gassing decreased pMMO activity by more than 30%. The steady-state cell density increased continuously over a 300-700 rpm range of agitation speed, whereas, the pMMO activity became maximal at 400 rpm. Also, the pMMO activity increased with the dilution rate up to 0.06 h-1 and remained constant thereafter. Maximal continuous pMMO productivity was, thus, achieved in Higgin's medium containing 10 μM Cu, 80 μM Fe, and 40 mM nitrate with an agitation speed of 500 rpm and a dilution rate of 0.06 h-1. Nitrogenase activity, on the other hand, increased over a feed medium copper concentration of 2-15 μM, falling sharply at 20 μM, and it exhibited a minimum at 20 mM when the feed medium nitrate concentration was varied. © 1992 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260400609
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  • 6
    Electronic Resource
    Electronic Resource
    Batch cultivation of Methylosinus trichosporium OB3b: II. Production of particulate methane monooxygenase (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 40 (1992), S. 151-157 
    Details
    ISSN: 0006-3592
    Keywords: methanotroph ; particulate methane monooxygenase ; nitrate depletion ; optimization of culture conditions ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The obligatory methanotroph, Methylosinus trichosporium OB3b, was studied to optimize the batch culture conditions for the formation of particulate methane monooxygenase (pMMO) in a nitrate minimal salts medium. The important medium components investigated were copper, carbon dioxide, and nitrate. The whole-cell specific pMMO activity decreased sharply with increasing copper concentrations in the range of 10-40 μM and remained constant upon further increases of the copper concentration to 120 μM. The cell growth rate (μ), on the other hand, decreased over the entire range (10-120 μM) of copper concentrations tested. When pMMO was produced in a bioreactor with an optimal initial copper concentration of 10 μM, M. trichosporium OB3b exhibited a much faster overall growth rate and a higher whole-cell propene epoxidation activity compared to our earlier study, in which soluble methane monooxygenase (sMMO) was produced with copper-deficient medium. The addition of external carbon dioxide to the bioreactor culture eliminated an initial lag period in the cell growth. When the standard culture medium nitrate concentration (10 mM) was depleted, the pMMO activity, but not the growth rate, decreased rapidly. The whole-cell specific pMMO activity could be maintained by subsequent supplementation of nitrate. A 4-fold higher initial culture medium nitrate concentration of 40 mM, however, resulted in slower cell growth and lower pMMO activity. These observations demonstrate that, in addition to affecting the exclusive production of pMMO, copper also has an important previously unrecognized role in enhancing the growth rate of M. trichosporium OB3b. They also indicate that for the optimal batch production of pMMO with the minimal medium under study, nitrate should be supplied intermittently during the course of cultivation until other culture medium components become growth-limiting.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260400121
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  • 7
    Electronic Resource
    Electronic Resource
    Batch cultivation of Methylosinus trichosporium OB3B: IV. Production of hydrogen-driven soluble or particulate methane monooxygenase activity (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 45 (1995), S. 229-238 
    Details
    ISSN: 0006-3592
    Keywords: methanotroph ; methane monooxygenase ; nitrogenase ; hydrogenase ; batch culture conditions ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Batch culture conditions were established for the formation of H2-driven whole-cell soluble or particulate methane monooxygenase (sMMO or pMMO) activity in the obligate methanotroph, Methylosinus trichosporum Ob3b, to expand its potential uses in groundwater bioremediation and the production of specific chemicals. Addition of either Ni and H2 to a nitrate-containing minimal salts growth medium or Ni and Mo to a nitrate-lacking growth medium (induces a nitrogenase that generates intracellular H2) markedly enhanced both the hydrogenase and the accompanying washed-cell H2-driven MMO activities of shake-flask cultured cells. For sMMO containing cells, H2 provided in vitro reducing power for the oxidation of chlorinated solvents such as chloroform and trichloroethylene. Cell cultivations under N2-fixing conditions in a 5-L bioreactor, however, required an initial nitrate concentration of at least 1 to 2 mM to achieve high biomass yields (5 to 7 g of dry cell wt/L) for cells producing H2-driven sMMO or pMMO activity. Elevation of the initial medium nitrate concentration to 20 mM shortened the culture time for pMMO producing cells by 40%, yet still generated an equivalent growth yield. High nitrate also shortened the culture time for sMMO containing cells by ∼25%, but it lowered their biomass yield by 26%. Upon storage for 5 weeks at room temperature, washed resting-state cells retained 90% and 70% of their H2-driven sMMO and pMMO activity, respectively. This makes their practical use quite feasible. © 1995 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260450307
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  • 8
    Electronic Resource
    Electronic Resource
    Batch cultivation of Methylosinus trichosporium OB3b: V. Characterization of poly-β-hydroxybutyrate production under methane-dependent growth conditions (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 49 (1996), S. 161-171 
    Details
    ISSN: 0006-3592
    Keywords: methanotroph ; poly-β-hydroxybutyrate ; methane monooxygenase ; batch culture conditions ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Methanotrophs have promising applications in the bioremediation of chlorinated hydrocarbons and in the production of a biopolymer, poly-β-hydroxybutyrate (PHB). Batch bioreactor culture conditions were studied for the accumulation of PHB by methane-grown Methylosinus trichosporium OB3b, and to evaluate the effect of PHB on the bacterial capacity to degrade trichloroethylene (TCE), a common groundwater contaminant. The PHB content of the washed and lyophilized cells was measured by gas chromatography (GC), after hydrochloric acid (HCl) propanolysis. A differential GC-based assay was developed for the monomer and the polymer of β-hydroxybutyrate utilizing 1% and 10% HCl (v/v) reaction mixtures, respectively. During bioreactor growth in a Cu-deficient modified Higgins' medium, the cells accumulated PHB upon depletion of nitrate. A biomass yield of 3.2 g dry wt/L and a PHB accumulation of ∼10% (w/w) were reached after 140 to 160 h, without adversely affecting the propene or TCE epoxidation specific rate given by whole cells containing soluble methane monooxygenase (sMMO). The TCE biotransformation capacity (∼0.25 mg TCE oxidized/mg dry cell wt) of resting cells containing ∼10% PHB was consistently ∼1.6-fold greater than that of cells containing only ∼2% PHB. Higher levels (〉10%) of accumulated PHB did not enhance this biotransformation capacity further. By replacing the bioreactor inlet air + CO2 mixture with pure O2 at ∼85 h of batch operation, a PHB accumulation of ∼45% was achieved after 160 h, but the whole-cell sMMO activity was markedly decreased. In contrast, cells grown in a 10 μM Cu-supplemented Higgins' nitrate minimal salts medium (particulate MMO formation) accumulated up to 50% PHB in only 120 h, coupled with a very high biomass yield of 18 g dry cell wt/L. High PHB accumulations above ∼20% by both the -Cu and the +Cu grown cells resulted in a decreased ratio of the electronic cell count to the absorbance at 660 nm, which is commonly used to monitor bacterial growth. © 1996 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    High-pressure - temperature bioreactor for studying pressure - temperature relationships in bacterial growth and productivity (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 31 (1988), S. 407-413 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Thermophilic organisms offer many potential advantages for biotechnological processes; however, realization of the promise of thermophiles will require extensive research on bacterial thermophily and high-temperature cultivation systems. This article describes a novel bioreactor suitable for precise studies of microbial growth and productivity at temperatures up to 260°C and pressures up to 350 bar. The apparatus is versatile and corrosion resistant, and enables direct sampling of both liquids and gases from a transparent culture vessel without altering the reaction conditions. Gas recirculation through the culture can be controlled through the action of a magnetically driven pump. Initial studies in this bioreactor of Methanococcus jannaschii, an extremely thermophilic methanogen isolated from a deep-sea hydrothermal vent, revealed that increasing the pressure from 7.8 to 100 bar accelerated the production of methane and cellular protein by this archaebacterium at 90°C, and raised the maximum temperature allowing growth from 90 to 92°C. Further increases in pressure had little effect on the growth rate at 90°C.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260310503
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  • 10
    Electronic Resource
    Electronic Resource
    High pressure-temperature bioreactor: Assays of thermostable hydrogenase with fiber optics (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 34 (1989), S. 1015-1021 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260340715
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