Publication Date:
2012-03-20
Description:
: Standard transcriptomics measures total cellular RNA levels. Our understanding of gene regulation would be greatly improved if we could measure RNA synthesis and decay rates on a genome-wide level. To that end, the Dynamic Transcriptome Analysis (DTA) method has been developed. DTA combines metabolic RNA labeling with standard transcriptomics to measure RNA synthesis and decay rates in a precise and non-perturbing manner. Here, we present the open source R / Bioconductor software package DTA . It implements all required bioinformatics steps that allow the accurate absolute quantification and comparison of RNA turnover. Availability: DTA is part of R / Bioconductor . To download and install DTA refer to http://bioconductor.org/packages/2.10/bioc/html/DTA.html Contact: schwalb@lmb.uni-muenchen.de
Print ISSN:
1367-4803
Electronic ISSN:
1460-2059
Topics:
Biology
,
Computer Science
,
Medicine
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