ISSN:
1617-4623
Keywords:
Streptococcal pyrogenic exotoxin A
;
Toxic shock syndrome toxin-1
;
Bacillus expression
;
Stability
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The genes encoding streptococcal pyrogenic exotoxin type A (SPE A) and staphylococcal toxic shock syndrome toxin-1 (TSST-1) were stably cloned and expressed in Bacillus subtilis. In the non-pathogenic Bacillus background, the recombinant speA clone expressed 32-fold more SPE A than the native streptococcus, and similarly, the recombinant plasmid harboring tst expressed 4-fold more TSST-1 in Bacillus than in the native Staphylococcus aureus. The Bacillus-derived products were secreted into the culture fluid, were resistant to proteolytic degradation and their biological activites mimicked native preparations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00330426
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