ISSN:
1600-0668
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Architecture, Civil Engineering, Surveying
,
Medicine
Notes:
Abstract The in vitro potency of house dust to induce cytokine response in A549 lung epithelial cells was studied. Dusts collected from carpet, bed, shelf and floor of a villa and an apartment by vacuuming were found to trigger the production of in-terleukin–8 (IL–8) and interleukin–6 (IL–6) in a dose–dependent manner, and the interleukin production was several–fold higher than of swine dust (used as a positive control). The IL–8 and IL–6 production of pure Escherichia coli lipopolysaccharide was significantly lower than of the dusts and a peptidoglycan-polysac-charide complex did not show any stimulatory effect at all. The lipopolysaccharide and peptidoglycan contents of the samples were determined by gas chromatography–tandem mass spectrometry analysis of, respectively, 3-hydroxy fatty acids and muramic acid; in addition, ergosterol was monitored for fungal bio-mass. The inflammatory properties of house dust upon inhalation may be reflected in its high potency to induce cytokine response in lung epithelial cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1600-0668.1999.00002.x
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