ISSN:
1662-0356
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Natural Sciences in General
,
Technology
Notes:
As a ceramic, silicon nitride (Si3N4) has been suggested as a biocompatible material incontact with bone. In another configuration, as a low pressure chemically vapor deposited(LPCVD) thin film, Si3N4 can also be used as the sensitive material in certain biosensorapplications. With the latter in mind, the biocompatibility of such films was investigated after thembeing modified with silane based self-assembled monolayers (SAMs) bearing functional end groupsof methyl (CH3), primary amine (NH2), and carboxyl (COOH) respectively. The SAM surfacemodifications provided a wide range of physiochemical properties including hydrophobic (CH3),hydrophilic (bare Si3N4), positively (NH2) and negatively charged (COOH). Specifically the celladhesion and proliferation, as well as the levels of alkaline phosphatase activity and osteocalcin,have been evaluated using the human osteoblast-like MG-63 cell line. It was observed thatattachment and spreading was pronounced on NH2 while suppressed on CH3. With time the cellsgrew to confluence on all chemistries, and the levels of osteocalcin normalized to total proteincontent varied as CH3 〉 Si3N4 〉 COOH 〉 NH2, but without significant differences. The highestspontaneous alkaline phosphatase activity was observed from cells grown on Si3N4 substrata
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/42/transtech_doi~10.4028%252Fwww.scientific.net%252FAST.53.122.pdf
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