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  • 1
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effects of single and mixed artificial infections by artichoke latent virus (ALV) and broad bean wilt virus isolate FA (BBWV-FA) on components of growth and yield of three globe artichoke lines were evaluated in an aphid-free glasshouse. Virus infections reduced plant vigour (length of leaves and height of the ramifications) and dry weight, increased dry matter percentage, lengthened harvest period, and induced significant losses in the number of harvested buds, which resulted from reduced production of ramifications. However, the magnitude of affected parameters depended on inherent line differences and the type of virus infection. The greatest reductions occurred with the ALV + BBWV-FA infection. In single infections ALV led to the more severe effects, especially on components of yield. The level of ALV coat protein, as determined by ELISA, indicated differences in titre between plants of three lines. Can line, which exhibited greater yield reduction than Cada or CBT lines, had the higher ALV virus titre. Conversely Cari was less susceptible to BBWV-FA infection than Cada. The detrimental effects of virus infection emphasize the need for virus control, and differences in responses among lines indicate the possibility of control by plant breeding.
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  • 2
    ISSN: 1432-2242
    Keywords: Key words Self-incompatibility ; S-haplotype ; S-Locus glycoprotein ; Immunochemical analysis ; Brassica oleracea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The self-incompatibility (SI) character in Brassica is controlled by the S locus which contains several genes. One of them, the SLG (S Locus Glycoprotein) gene encodes a soluble glycoprotein expressed in the stigma. We used antibodies directed against SLGs and a combination of isoelectric focusing (IEF) and immunoblotting methods to identify S haplotypes, the allelic forms of the S locus, in commercial and open-pollinated varieties of broccoli and romanesco. We found 23 class-I and three class-II S haplotypes among the 199 plants analysed. Nevertheless, for a few plants, SLGs were not detected by the antibodies and these plants, designated Hw for “white pattern” haplotypes, were apparently homozygous at the S locus. Diallel crosses between Hw plants revealed the existence of four different Hw haplotypes. Several hypotheses are discussed to explain the non-recognition of the SLG products in these Hw haplotypes. The data of the present study were compared with those obtained in a previous investigation carried out on cauliflower. As in cauliflower, we observed a high frequency of the sx haplotype and a great variability in the strength of the SI phenotype for sx plants (in the homozygous or heterozygous state). For both broccoli and romanesco, about 50% of the plants presented a SI phenotype strong enough to be exploited for hybrid production.
    Type of Medium: Electronic Resource
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