ISSN:
1573-0832
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Sclerotia and mycelium ofPhymatotrichum omnivorum were exposed to anhydrous ammonia (NH3) and then observed with an electron microscope in order to determine the effects of the NH3 treatment on the fungal membranes. Sclerotia were exposed to four rates of NH3: 28, 56, 84, and 112μg NH3/ml of air for 24 hours. At 28μg/ml, the plasmalemma became wavy and the mitochondrial cristae began to swell and disperse. At 56μg NH3/ml the plasmalemma showed breakage and formation of vesicles, and all other membrane systems within the cell were broken and distorted. All membranes were totally disrupted and no organelles were recognizable at 84μg NH3/ml. Mycelium was exposed to 2, 4, 8, 20, and 40μg NH3/ml for one minute. Damage to cell membranes was not observed at NH3 conc. up to 4μg/ ml. At 8μg NH3/ml the plasmalemma was broken and the mitochondria were disrupted. At 20μg/ ml and above, all internal organization was destroyed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01837194
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