ISSN:
1572-9729
Keywords:
accelerostat
;
biotransformation
;
co-metabolism
;
Corynebacterium glutamicum
;
demeton-S-methyl
;
growth rate
;
organophosphorus compounds
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Energy, Environment Protection, Nuclear Power Engineering
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract The organophosphorous pesticide, demeton-S-methyl was transformed byCorynebacterium glutamicum in co-metabolism with more readilydegradable substrates. Glucose, acetate and fructose were tested as growth substrates, and the highest demeton-S-methyl biotransformation average rate (0.78 mg l-1 h-1) and maximum instantaneous rate (1.4 mg l-1 h-1) were achieved on fructose. This higher efficiency seems to be linked to the atypical behavior of C. glutamicum grown on fructose, characterized by a prolonged period of accelerating growth instead of a constant growth rate observed on glucose or acetate. More precisely, for growth rates in the 0.1–0.4 h-1 range, a direct coupling between the specific demeton-S-methyl consumption rate and the growth rate was demonstrated on fructose during batch –, steady state continuous – or continuous cultures with a controlled transient growth rate (accelerostat technology). The demeton-S-methyl biotransformation was more favoured during an acceleration phase of the growth rate.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1011682714928
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