ISSN:
0173-0835
Keywords:
Two-dimensional polyacrylamide gel electrophoresis
;
Proteins
;
RNA
;
Maternal/paternal inheritance
;
Mouse
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
A novel strategy for identifying proteins which reveal maternal or paternal inheritance in the mouse is presented. Using two-dimensional electrophoresis we investigated protein expression patterns of adult liver and different embryonic and extraembryonic tissue in C57BL/6Crl and in DBA/2Crl mice, as well as in their reciprocal hybrids. We found three groups of protein spots which showed maternal or paternal inheritance of quantitative variations. These proteins were characterized by N-terminal or internal amino acid sequencing, by determination of the amino acid composition, by glycoprotein staining and RNA expression analysis. The three proteins identified were: α-enolase, cyclophilin and β-group hemoglobins. The parental effects observed for α-enolase and cyclophilin were found to be due to parent-specific post-translational modifications of these proteins. For the β-group hemoglobins our results suggested parental effects on the transcriptional level.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.11501601137
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