ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
Crystals have been grown of a mannose-specific lectin from bluebell (Scilla campanulata) bulbs in a form suitable for X-ray diffraction studies. The crystals, which diffract to high resolution, grew in hanging drops by vapour diffusion, equilibrating with a solution of 70% saturated ammonium sulfate at pH 4.7–4.8 at 293 K, in the absence of any mannose saccharides. Crystals are orthorhombic, P21212, with unit-cell dimensions a = 70.78, b = 93.69, c = 46.92 Å. The functional lectin molecule is organized as a tetramer of four identical 14 kDa subunits, with only two subunits in the asymmetric unit. Data to 1.86 Å resolution have been recorded and the structure determined by the molecular replacement method.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444996006889
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