ALBERT

Beschreibung: The effects of gravity on the dynamic performance of structural control actuators and sensors are dual forms of an additive perturbation that can attenuate or amplify the device response (input or output). The modal modeling of these perturbations is derived for the general case of arbitrarily oriented devices and arbitrarily oriented planes of deformation. A nondimensional sensitivity analysis to identify the circumstances under which the effects of gravity are important is presented. Results show that gravity effects become important when the product of the ratio of the normalized modal slope and the modal displacement is comparable to the ratio of the gravitational acceleration and the product of the beam length and the squared eigenfrequency for a given mode.

Beschreibung: The effects of gravity on the actuators, sensors and structural plant of a controlled flexible structure are investigated. These influences include gravity stiffening, gravity induced deformations, gravity influence on inertial sensors and actuators, and the interaction with the suspension system. For each of the four influences, a simplified model is derived, from which the appropriate non-dimensional parameter can be identified, and the magnitude of the influence estimated. The construction of a detailed numerical model, which includes the four gravity influences, is outlined. This modeling procedure is then applied to a simplified model of the Middeck Active Control Eperiment (MACE). The influence on the poles and input-output transfer function is examined for several variations in the plant. The modeling procedure is also applied to the MACE experimental hardware. Experimental and model-based transfer functions are compared to demonstrate the significant improvement by incorporating gravity effects.

Beschreibung: Background Corynebacterium glutamicum is a high-GC Gram-positive soil bacterium of great biotechnological importance for the production of amino acids. To facilitate the rational design of sulphur amino acid-producing strains, the pathway for assimilatory sulphate reduction providing the necessary reduced sulfur moieties has to be known. Although this pathway has been well studied in Gram-negative bacteria like Escherichia coli and low-GC Gram-positives like Bacillus subtilis, little is known for the Actinomycetales and other high-GC Gram-positive bacteria. Results The genome sequence of C. glutamicum was searched for genes involved in the assimilatory reduction of inorganic sulphur compounds. A cluster of eight candidate genes could be identified by combining sequence similarity searches with a subsequent synteny analysis between C. glutamicum and the closely related C. efficiens. Using mutational analysis, seven of the eight candidate genes, namely cysZ, cysY, cysN, cysD, cysH, cysX, and cysI, were demonstrated to be involved in the reduction of inorganic sulphur compounds. For three of the up to now unknown genes possible functions could be proposed: CysZ is likely to be the sulphate permease, while CysX and CysY are possibly involved in electron transfer and cofactor biosynthesis, respectively. Finally, the candidate gene designated fpr2 influences sulphur utilisation only weakly and might be involved in electron transport for the reduction of sulphite. Real-time RT-PCR experiments revealed that cysIXHDNYZ form an operon and that transcription of the extended cluster fpr2 cysIXHDNYZ is strongly influenced by the availability of inorganic sulphur, as well as L-cysteine. Mapping of the fpr2 and cysIXHDNYZ promoters using RACE-PCR indicated that both promoters overlap with binding-sites of the transcriptional repressor McbR, suggesting an involvement of McbR in the observed regulation. Comparative genomics revealed that large parts of the extended cluster are conserved in 11 of 17 completely sequenced members of the Actinomycetales. Conclusion The set of C. glutamicum genes involved in assimilatory sulphate reduction was identified and four novel genes involved in this pathway were found. The high degree of conservation of this cluster among the Actinomycetales supports the hypothesis that a different metabolic pathway for the reduction of inorganic sulphur compounds than that known from the well-studied model organisms E. coli and B. subtilis is used by members of this order, providing the basis for further biochemical studies.