Publication Date:
2024-02-15
Description:
Phaeodactylum tricornutum strain CCAP 1052/1A was cultivated at 6°C and 15°C under controlled conditions (32 salinity, F/2 medium, 400 µatm pCO2, 100 µmol photons m-2 s-2 light intentsity 16:8 light:dark cycle) in semi-continous batch cultures. We assessed the carbonate chemistry (pH, total alkalinity, dissolved inorganic carbon), growth rates, particulate organic carbon and nitrogen (POC and PON), chlorophyll a quota (Chl a), POC:PON ratios, Chl a:POC ratios as well as production rates at both acclimation temperatures. Additionally, we performed biological invivo assays to measure rates of gross photosynthetic oxygen release, gross photosynthetic carbon uptake, respiratory oxygen uptake and respiratory carbon release using membrane-inlet mass-spectrometry. Assays were performed in photosynthesis-irradiance-(PI-)curves of increasing light intensity (0, 50, 150, 250, 400 µmol photons m-2 s-2). First rates were measured under acclimation temperature (6°C and 15°C), directly afterwards, the assay temperature was abruptly shifted to 15°C or 6°C, respectively, and the PI-curve measurement was repeated, so that 6°C acclimated cells were measured at 15°C and 15°C acclimated cells were measured at 6°C. Q10 factors were calculated from acclimated cells und the respective temperature shift. Photosynthetic and respiratory quotients were calculated for acclimated cells as well as after the abrupt temperature shift. PI-parameters, i.e. maximum photosynthesis rate, light use efficiency and light saturation index were calculated. All experiments were performed in laboratories at the Alfred-Wegener-Institute Bremerhaven.
Keywords:
Alkalinity, total; Calculated; Calculation according to Rehder et al. (2023); Calculation according to Rokitta & Rost (2012); Carbon, inorganic, dissolved; Carbon, organic, particulate, per cell; Carbon, organic, particulate/Nitrogen, organic, particulate ratio; Chlorophyll a/particulate organic carbon ratio; Chlorophyll a per cell; Coulter Counter (Beckman Coulter); Date/time end, experiment; Date/time start, experiment; Diatom; Elemental analyzer, EuroVector, EA 3000; Experiment; Factor quantifying temperature dependent change of rates of processes; Generation; Gross photosynthetic carbon uptake rate, per chlorophyll a; Gross photosynthetic oxygen release rate, per chlorophyll a; Growth rate; Laboratory; Laboratory experiment; Laboratory fluorometer, Turner, Trilogy; Light saturation index; Light use efficiency; Maximum photosynthetic carbon uptake rate, per chlorophyll a; Maximum photosynthetic oxygen release rate, per chlorophyll a; Measured with colorimetric assay on QuAAtro continuous segmented flow analyzer (Seal Analytical); Medium; Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime; Nitrogen, organic, particulate, per cell; Particulate organic carbon production per cell; pH; Phaeodactylum tricornutum; Photosynthesis; Photosynthetic quotient; Physiological performance; Phytoplankton; Respiration; Respiratory carbon release rate, per chlorophyll a; Respiratory oxygen uptake rate, per chlorophyll a; Respiratory quotient; Salinity; Sample ID; Sampling date/time, experiment; Species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Strain; Temperature; Titration analyzer, Schott Instruments, TitroLine alpha plus; Treatment: light:dark cycle; Treatment: light intensity; Treatment: temperature; Type of study
Type:
Dataset
Format:
text/tab-separated-values, 736 data points
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