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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of somatic hybrids of potato by use of RAPD markers and isozyme analysis (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 93 (1995), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Electrofusion of mesophyll protoplasts from two male sterile dihaploid Solanum tuberosum genotypes. DHAK-11 and DHAK-33, was performed. Selection of putative fusion products was based on vigorous callus growth. Regeneration of rooted putative hybrid plants was scored 14 weeks after fusion. Characterization of hybrids was performed by use of morphological assessment, random amplified polymorphic DNA (RAPD), and cytological and isozyme analysis. The rate of regenerated hybrids from callus was ca 6%. Of the putative hybrids, 45% were confirmed as true hybrids. Morphological assessment of the putative hybrids revealed that tetraploid and neartetraploid hybrids were vigorous plants with intermediate characteristics between the two parental phenotypes in respect to internode length, leaf size and shape, and purple pigmentation on the abaxial side of the leaves. Near-hexaploid hybrids were slender plants with small leaves and short petioles. Selected RAPD primers showed unique marker bands for the two parental genotypes. Hybrid plants revealed the unique marker bands from both parents. A total of 53 randomly chosen decamer primers were tested and 26 primers (49%) detected polymorphism between the two dihaploid parentals. Two primers revealed that one parental marker band was missing in two aneuploid hybrids. However, of 51 putative hybrids, a double test with two independently chosen primers showed unequivocally the hybrid character of 23 plants. The ploidy level of the hybrids was analysed by chromosome numbers in root tip cells and by number of guard cell chloroplasts. A strong correlation between the chromosome number and the number of chloroplasts was obtained. The hybrid nature of all RAPD-verified hybrids was confirmed by isozyme analysis with malate dehydrogenase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1995.tb02240.x
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  • 2
    Electronic Resource
    Electronic Resource
    On the influence of shell structure and matrix element fluctuations on the pairing correlation in nuclei (1967)
    Springer
    The European physical journal 202 (1967), S. 182-189 
    Details
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The uniform model for the nuclear pairing-force problem is extended to take into account the effect of fluctuations in nucleon orbital level densityρ and in pairing matrix elementsG vv′. Simple formulas for the average dependence of level density on energy are presented, and alternative ways of estimating effects of shell bunching on the pairing correlation are derived. It is argued also that the pairing constantG might be systematically overestimated in the usual BCS numerical calculations; a semiconstant pairing force with diagonal elements larger than off-diagonal is thus suggested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01331208
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  • 3
    Electronic Resource
    Electronic Resource
    Gamma and alpha decay from the 2.1-msec isomer213mRa (1977)
    Springer
    The European physical journal 282 (1977), S. 124-124 
    Details
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01881015
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  • 4
    Electronic Resource
    Electronic Resource
    Analysis of somatic hybrids between two sterile dihaploid Solanum tuberosum L. breeding lines. Restoration of fertility and complementation of G. pallida Pa2 and Pa3 resistance (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 403-410 
    Details
    ISSN: 1432-2242
    Keywords: Globodera pallida ; Solanum tuberosum L ; Solanum vernei ; Intraspecific somatic hybridization ; Fertility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fourteen somatic hybrids generated by electrofusion of mesophyll protoplasts from a non-flowering dihaploid S. tuberosum clone, DHAK-11, and a male-sterile dihaploid clone S. tuberosum, DHAK-33, were grown in the greenhouse and subjected to morphological assessments and tests for fertility and resistance to the white potato cyst nematode Globodera pallida pathotypes Pa2 and Pa3. The ploidy level of the hybrids ranged from 38 to 63 chromosomes. All hybrids developed flowers with violet petals except for one, hy-56, that possessed red petals. The colour of the tuber skin was purple in all hybrids except in hy-56 where the tuber skin was red. All of the hybrids were female fertile and generated viable seeds. Near-tetraploid hybrids produced the highest number of seeds per fruit and these seeds had a normal size. Hybrids with 58 or more chromosomes produced smaller seeds and less seeds per fruit. The germination frequency of the seeds was not influenced by the chromosome number of the hybrids. Pollen viability was determined and the male fertility of three hybrids was tested. Pollination with these three hybrids gave rise to fruit development, but only one produced viable seeds. The hybrids were tested for resistance to G. pallida pathotypes Pa2 and Pa3. A high level of resistance to Pa3, inherited from one parental clone, DHAK-11, and a high level of resistance to Pa2, inherited from the other parental clone, DHAK-33, was combined in four hybrids. These results demonstrate, that protoplast fusion is an efficient method for restoring the fertility of somatic hybrids generated from sterile parent clones, and is a powerful procedure for the complementation of multigenetic disease resistance traits in potato breeding lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223686
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  • 5
    Electronic Resource
    Electronic Resource
    Regeneration and analysis of interspecific asymmetric potato –Solanum ssp hybrid plants selected by micromanipulation or fluorescence-activated cell sorting (FACS) (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 41-49 
    Details
    ISSN: 1432-2242
    Keywords: Key words Protoplasts ; Solanum tuberosum ; Solanum ssp. donor ; RAPD ; Chromosome elimination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Recipient protoplasts from three Solanum tuberosum genotypes, cv ‘Folva’ (2n=4x=48), cv ‘Matilda’ (4n) and ‘161 : 14’ (2n), were electrofused with X-ray-irradiated donor protoplasts from two wild species S. spegazzinii (2n) or S. microdontum×S. vernei (2n). Prior to fusion, protoplasts were fluorescence-labelled with either fluorescein diacetate or scopoletin. Fusion products were identified by dual fluorescence and selected by micromanipulation or fluorescence-activated cell sorting (FACS). All putative hybrid plants were analysed by the random amplified polymorphic DNA (RAPD) technique. Our analysis demonstrates that each asymmetric hybrid plant has an individual and stable profile of donor-specific RAPD bands. The irradiation of donor protoplasts hampered the growth of selected heterofusion products in a dose-dependent way. Irradiation resulted in donor chromosome elimination, but not in a dose dependent way, in the tested interval. In asymmetric hybrids with the S. spegazzinii donor 33–68% of the donor-specific RAPD bands were missing, indicating a similar level of chromosome elimination. In asymmetric hybrid plants with the S. microdontum×S. vernei donor 74–95% of the donor RAPD bands were missing. Chromosome countings revealed that these hybrids had chromosome numbers equal to or below the chromosome numbers found in the tetraploid recipients. This is the first time that highly asymmetric hybrid plants between two tetraploid potato recipients and the donor S. microdontum×S. vernei have been obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050530
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  • 6
    Electronic Resource
    Electronic Resource
    Analysis of somatic hybrids between two sterile dihaploid Solanum tuberosum L. breeding lines. Restoration of fertility and complementation of G. pallida Pa2 and Pa3 resistance (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 403-410 
    Details
    ISSN: 1432-2242
    Keywords: Key words  Globodera pallida ; Solanum tuberosum L. ; Solanum vernei ; Intraspecific somatic hybridization ; Fertility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Fourteen somatic hybrids generated by electrofusion of mesophyll protoplasts from a non-flowering dihaploid S. tuberosum clone, DHAK-11, and a male-sterile dihaploid clone S. tuberosum, DHAK-33, were grown in the greenhouse and subjected to morphological assessments and tests for fertility and resistance to the white potato cyst nematode Globodera pallida pathotypes Pa2 and Pa3. The ploidy level of the hybrids ranged from 38 to 63 chromosomes. All hybrids developed flowers with violet petals except for one, hy-56, that possessed red petals. The colour of the tuber skin was purple in all hybrids except in hy-56 where the tuber skin was red. All of the hybrids were female fertile and generated viable seeds. Near-tetraploid hybrids produced the highest number of seeds per fruit and these seeds had a normal size. Hybrids with 58 or more chromosomes produced smaller seeds and less seeds per fruit. The germination frequency of the seeds was not influenced by the chromosome number of the hybrids. Pollen viability was determined and the male fertility of three hybrids was tested. Pollination with these three hybrids gave rise to fruit development, but only one produced viable seeds. The hybrids were tested for resistance to G. pallida pathotypes Pa2 and Pa3. A high level of resis-tance to Pa3, inherited from one parental clone, DHAK-11, and a high level of resistance to Pa2, inherited from the other parental clone, DHAK-33, was combined in four hybrids. These results demonstrate, that protoplast fusion is an efficient method for restoring the fertility of somatic hybrids generated from sterile parent clones, and is a powerful procedure for the complementation of multi-genetic disease resistance traits in potato breeding lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050142
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  • 7
    Electronic Resource
    Electronic Resource
    Analysis of the plastome and chondriome origin in plants regenerated after asymmetric Solanum ssp. protoplast fusions (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 336-343 
    Details
    ISSN: 1432-2242
    Keywords: Key words Potato ; Mitochondria ; Chloroplast ; Asymmetric hybrids ; Cybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Protoplasts from potato cultivars used as recipient parents were fused with irradiated protoplasts from wild Solanum donor species. Regenerated plants were analysed by RAPDs to identify hybrids. Irradiation of donor protoplasts with ionizing irradiation induced a broad range of donor nuclear DNA elimination in the asymmetric hybrids. Usage of chloroplast (cp)- and mitochondrial (mt)-specific PCR markers made it possible to trace the different origins of the cp genome in seven fusion combinations, as well as the mt genomes in two fusion combinations. Regenerated plants with recipient nucleus and plastome markers from the donors were found in six of the seven analysed fusion combinations. Protoplast fusion has generated novel mt genome combinations consisting of different portions of the mt genomes from the fusion partners. Selection of heterofusion products based on fluorescence markers is an efficient method to obtain asymmetric Solanum hybrids and cy- brids from most fusion combinations. Possible models for cybrid formation are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051488
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  • 8
    Electronic Resource
    Electronic Resource
    Bandcrossing inK≠0 bands (1979)
    Springer
    The European physical journal 292 (1979), S. 271-273 
    Details
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract It is shown that in the crossing of two bands withK≠0 in deformed nuclei, two new effects are observed that are not seen in the crossing ofK=0 bands; namely, a)I critical at which the bands cross is lowered and b) Band crossing occurs at different values ofI crit for oddI (orI+1/2 in oddA) and evenI (orI+1/2). These predictions are shown to be observed in the odd-A rare-earth nuclei where data are available to high spin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01547472
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  • 9
    Electronic Resource
    Electronic Resource
    Gamma and Alpha decay from the 2.1-msec isomer213m Ra (1976)
    Springer
    The European physical journal 279 (1976), S. 301-311 
    Details
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The isomer213mRa was produced by the reaction209Bi(10B, 6n), as well as by bombardments of12C on Pb isotopes,14N on Bi, and16O on natural Hg, with projectile energies in the range 60–100 MeV. The isomer decays with a halflife of 2.1 ms both via gamma internal transitions and via alpha branching to levels in209Rn. A level scheme is proposed in which the isomeric state is assigned as either 17/2− or 13/2+ with shell-model configurations either of theh 9/2 protons coupled to ap 1/2 neutron hole, or of an uncoupledi 13/2 neutron hole. On the basis of alpha decay rate predictions from the new Fliessbach theory, the 17/2− isomeric assignment is to be preferred. Phys. Abstracts classifications 4.200–4.375 E [Radioactivity213m Ra [from209Bi(10B, 6n),204-6Pb(12C, 3n–5n) etc.,E=60–100 MeV]; measuredE α ,E γ ,I α ,I γ ,I x ,γγ-coin,xγ-coin,α K,T 1/2.213Ra,209Rn deduced levels,J, π,γ-multipolarity.]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01408303
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  • 10
    Electronic Resource
    Electronic Resource
    Nuclear rotational population patterns in heavy-ion scattering and transfer reactions (1991)
    Springer
    The European physical journal 339 (1991), S. 141-145 
    Details
    ISSN: 1434-601X
    Keywords: 25.70.Cd ; 27.90.+b
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A model of239Pu with decoupled neutron is used for theoretical calculations of rotational population patterns in heavy ion inelastic scattering and one-neutron transfer reactions. The system treated is90Zr on239Pu at the near-barrier energy of 500 MeV and backscattering angles of 180° and 140°. The influence of the complex nuclear optical potential is seen to be very strong, and the Nilsson wave function of the odd neutron produces a distinctive pattern in the transfer reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01282943
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