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  • 1
    Electronic Resource
    Electronic Resource
    Conformational kinetics in liquid n-butane by transition path sampling (2001)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 115 (2001), S. 7285-7292 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: A recent method for the study of transition states, transition path sampling, is applied to a classical problem of first order dynamics, the conformational transitions trans–gauche in pure liquid n-butane. Not only can the rate constant be calculated, but an ensemble of transition states can be extracted and analyzed as well. The results of the rate constant and the different distributions at the transition state are compared to those obtained from an equilibrium molecular dynamics simulation of the same system. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1405445
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  • 2
    Electronic Resource
    Electronic Resource
    Organometalloidal derivatives of the transition metals. 32. Base-induced migrations from iron to the .eta.5-indenyl ligand in the complexes (.eta.5-C9H7)Fe(CO)2R (R = silyl, oligosilyl) (1992)
    s.l. : American Chemical Society
    Organometallics 11 (1992), S. 3139-3143 
    Details
    ISSN: 1520-6041
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/om00045a034
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  • 3
    Electronic Resource
    Electronic Resource
    Involvement of Nha1 antiporter in regulation of intracellular pH in Saccharomyces cerevisiae (1999)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 171 (1999), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The Nha1 antiporter is involved in regulation of intracellular pH in Saccharomyces cerevisiae. We report that deletion of the NHA1 gene resulted in an increase of cytoplasmic pH in cells suspended in water or acidic buffers. Addition of KCl or NaCl to exponentially growing cells lowered the internal pH but the difference between cells with or without NHA1 was maintained. Addition of KCl to starved cells resulted in much higher alkalinization of cytoplasmic pH in a strain lacking Nha1p compared to the wild-type or Nha1p-overexpressing strains. The H+/K+(Na+) exchange mechanism of Nha1p was confirmed in reconstituted plasma membrane vesicles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1999.tb13428.x
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  • 4
    Electronic Resource
    Electronic Resource
    Measurement of kinetic parameters relevant to the operation of an electron-beam initiated atomic iodine laser (1985)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 57 (1985), S. 2431-2436 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Parameters relevant to the operation of an electron-beam initiated atomic iodine laser, which would lase on the transition 5 2P1/2 (I*)→5 2P3/2 (I), have been measured by pulse radiolysis. Kinetic data for the parent compound quenching of I* were obtained by variation of the parent compound pressure at constant buffer gas pressure and observing I* decay rates versus time. Deactivation rates for perfluoroalkyl iodides were found to be much lower than for the corresponding alkyl iodides. Values obtained (in cm3 molec−1 s−1) are as follows: CH3I, (2.0±0.1)×10−13; C2H5I, (5.0±0.3)×10−13; CF3I, (8.8±0.3)×10−16; C2F5I, (9.7±1.0)×10−15; n-C3F7I, (2.5±0.2)×10−15; i-C3F7I, (1.7±0.1)×10−15; n-C4F9I, (1.8±0.1)×10−14. The extent of population inversion was investigated by measuring initial excited state and ground state atomic iodine concentrations, which gives the branching ratio [I*]/[I]. Values obtained are as follows: CH3I, 2.7; CF3I, 3.8; C2F5I, 2.7; i-C3F7I, 3.2; n-C4F9I, 1.8. Comparing both branching ratios and lifetimes, it is seen that CF3I is the most promising candidate for an electron beam initiated atomic iodine laser.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.334351
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  • 5
    Electronic Resource
    Electronic Resource
    MAJOR HISTOCOMPATIBILITY COMPLEX I POLYMORPHISM IN A CETACEAN: THE GRAY WHALE (ESCHRICHTIUS ROBUSTUS) (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Marine mammal science 20 (2004), S. 0 
    Details
    ISSN: 1748-7692
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Vertebrate Major Histocompatibility Complex-I (Mbc-I) proteins bind and display self and foreign peptides on the cell surface. Mbc-I polymorphism is considered critical for eliciting immune responses to a diversity of antigens, and thus, for the health and conservation of a species. Based on restriction fragment length polymorphism it was concluded that whales generally have low Mbc-I polymorphism. This was attributed to the weak pathogenic pressure in aquatic habitats. Since gray whales' habits might favor their encounter with diverse pathogens, resulting in selection pressures for Mbc variability, we searched for functional polymorphism of gray whale's Mbc-I exon 2 sequences that encode the α-1 protein domain of the antigen-binding region. We sequenced twelve Mbc-I exon 2 clones from each of seven gray whales. Most obtained sequences were similar to functional artiodactyl sequences, and their reconstructed phylogeny consistently showed three whale Mbc-I exon 2 sequence clusters (A, B, and C). Sequences within a given cluster and from distinct clusters, showed a greater number of non-synonymous than synonymous substitutions, that commonly shifted the physiochemical properties of the involved residue, suggesting the existence of a diverse repertoire of Mbc-I antigen recognition sites in gray whales, and that selection maintains gray whale's Mbc-I allelic diversity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1748-7692.2004.tb01155.x
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  • 6
    Electronic Resource
    Electronic Resource
    FIRST RECORD OF THE PYGMY BEAKED WHALE MESOPLODON PERUVIANUS IN THE NORTH PACIFIC (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Marine mammal science 8 (1992), S. 0 
    Details
    ISSN: 1748-7692
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1748-7692.1992.tb00058.x
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  • 7
    Electronic Resource
    Electronic Resource
    Identification of a 20-kDa protein with calcium uptake transport activity. Reconstitution in a membrane model (1994)
    Springer
    Journal of bioenergetics and biomembranes 26 (1994), S. 555-562 
    Details
    ISSN: 1573-6881
    Keywords: Calcium uptake ; uniporter ; mitochondria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract This paper presents results of experiments designed to further purify the membrane system involved in mitochondrial calcium transport. A partially purified extract, which transported calcium with a specific activity of 1194 nmol45Ca2+/mg protein/5 min, was used to obtain mouse hyperimmune serum. This serum inhibited calcium uptake both in mitoplasts and in vesicles reconstituted with mitochondrial proteins containing cytochrome oxidase. Western blot analysis of the semipurified fraction showed that the serum recognized specifically two antigens of 75 and 20 kDa. Both antibodies were purified by elution from the nitrocellulose sheets and their inhibition capacity was analyzed. The antibody that recognized the 20-kDa protein produced a higher degree of inhibition than the other one.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00762740
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  • 8
    Electronic Resource
    Electronic Resource
    The electronic structure of oxide-supported tungsten oxide catalysts as studied by UV spectroscopy (1998)
    Springer
    Catalysis letters 56 (1998), S. 29-33 
    Details
    ISSN: 1572-879X
    Keywords: tungsten oxides ; UV-vis spectra ; electronic structure ; HDS catalysts ; SCR catalysts ; oxidation catalysts ; titania ; alumina ; zirconia ; titania–alumina ; titania–zirconia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The UV spectra of tungsten oxide catalysts supported on alumina, titania and zirconia, and on titania–alumina and titania–zirconia mixed oxides are reported and discussed. Evidence is provided for the different electronic structure of supports and catalysts, which could affect the behavior of the tungsten oxide centres in the different cases. On alumina, tungsten oxide centres are “isolated” by the insulating support, while on titania-based materials they are likely in electronic contact with each other and with Ti centres through the support conduction band. In the case of WOx2013;ZrO2, the 5d levels of tungsten ions fall just below of the lower energy limit of the support conduction band.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1019076121915
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  • 9
    Electronic Resource
    Electronic Resource
    Inactivation of Mitochondrial Permeability Transition Pore by Octylguanidine and Octylamine (2000)
    Springer
    Journal of bioenergetics and biomembranes 32 (2000), S. 193-198 
    Details
    ISSN: 1573-6881
    Keywords: Mitochondria ; octylguanidine ; octylamine ; carboxyatractyloside ; permeability transition ; kidney mitochondria ; nonspecific pore ; calcium ; mitochondrial calcium ; mitochondrial membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Mitochondrial permeability transition occurs through a Ca2+-dependent opening of atransmembrane pore, whose identity has been attributed to that of the adenine nucleotide translocase(ANT). In this work, we induced permeability transition by adding 0.5 μM carboxyatractyloside.The process was evaluated analyzing Ca2+ efflux, a drop in transmembrane electric gradient,and swelling. We found that the amphiphyllic cations octylguanidine and octylamine, at theconcentration of 100 μM, inhibited, almost completely, nonspecific membrane permeability.Hexylguanidine, hexylamine, as well as guanidine chloride and hydroxylamine failed to doso. The inhibition was reversed after the addition of 40 mM Li+, Na+ K+,Rb+, or Cs+; K+ wasthe most effective. We propose that the positive charge of the amines interact with negativecharges of membrane proteins, more likely the ADP/ATP carrier, while the alkyl chain penetratesinto the hydrophobic milieu of the inner membrane, fixing the reagent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005516115189
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  • 10
    Electronic Resource
    Electronic Resource
    Calcium transport sensitive to ruthenium red in cytochrome oxidase vesicles reconstituted with mitochondrial proteins (1991)
    Springer
    Journal of bioenergetics and biomembranes 23 (1991), S. 889-902 
    Details
    ISSN: 1573-6881
    Keywords: Ca2+ transport ; liposomes ; ruthenium red ; mitochondria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract We describe a calcium transport that is sensitive to ruthenium red in liposomes reconstituted with mitochondrial extracts. This system is able to build an internally negative membrane potential, which allows the electrogenic influx of Ca2+ and Sr2+. Proteins with molecular weights higher than 35 kDa were incorporated to the vesicles, and enhanced the accumulation of the cation in an energy-dependent fashion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00786007
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