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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of a novel obligate anaerobe that ferments agar (1994)
    Springer
    Archives of microbiology 162 (1994), S. 395-400 
    Details
    ISSN: 1432-072X
    Keywords: Anaerobe ; Marine algae ; Agar degradation ; Acetate ; Ethanol ; Agarose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An agar-fermenting bacterium (strain 16AV) was isolated from the sediment of an abattoir effluent waste pond. The cells were curved rods, non-motile, gramnegative, and did not form endospores. Old cultures produced spherical bodies that were not refractile and did not survive pasteurization at 80°C for 5 min. Spherical cells were viable after at least 10 months storage at room temperature. The bacterium was metabolically restricted and grew only on agar, agarose, galactose, and cellobiose. No inorganic electron acceptors were reduced. Acetate and ethanol were produced as end products. Strain 16AV grew between 20 and 37°C, with optimum growth occurring at 28°C. Growth occurred in medium containing NaCl from 0 to 15 g l-1, with an optimum between 2.5 and 5 g l-1. The DNA base composition was 39.5 mol% G+C. 16S rDNA sequence analysis showed that strain 16AV falls within the radiation of members of the genus Clostridium and related taxa. This is the first description of an obligate anaerobe capable of degrading agar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00282103
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of a novel obligate anaerobe that ferments agar (1994)
    Springer
    Archives of microbiology 162 (1994), S. 395-400 
    Details
    ISSN: 1432-072X
    Keywords: Key words     Anaerobe ; Marine algae ; Agar degradation ; Acetate ; Ethanol ; Agarose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract      An agar-fermenting bacterium (strain 16AV) was isolated from the sediment of an abattoir effluent waste pond. The cells were curved rods, non-motile, gram-negative, and did not form endospores. Old cultures produced spherical bodies that were not refractile and did not survive pasteurization at 80°C for 5 min. Spherical cells were viable after at least 10 months storage at room temperature. The bacterium was metabolically restricted and grew only on agar, agarose, galactose, and cellobiose. No inorganic electron acceptors were reduced. Acetate and ethanol were produced as end products. Strain 16AV grew between 20 and 37°C, with optimum growth occurring at 28°C. Growth occurred in medium containing NaCl from 0 to 15 g l–1, with an optimum between 2.5 and 5 g l–1. The DNA base composition was 39.5 mol% G+C. 16S rDNA sequence analysis showed that strain 16AV falls within the radiation of members of the genus Clostridium and related taxa. This is the first description of an obligate anaerobe capable of degrading agar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050156
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of Aquamicrobium defluvii gen. nov. sp. nov., a thiophene-2-carboxylate-metabolizing bacterium from activated sludge (1998)
    Springer
    Archives of microbiology 169 (1998), S. 293-302 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsAquamicrobium defluvii ; Thiophene-2-carboxylic acid ; Thiophene-2-carboxy-CoA ligase ; Thiophene-2-carboxyl-CoA thioesterase ; Thiophene-2-carboxyl-CoA dehydrogenase ; Thiosulfate ; Sulfur ; Molybdenum ; Nitrate ; Nitrite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A gram-negative bacterium was isolated from activated sewage sludge with thiophene-2-carboxylate as the sole source of carbon and with nitrate as an electron acceptor. The isolate, strain NKK, was a motile, oxidase- and catalase-positive, rod-like bacterium with a G+C content of 61.7 mol%. Besides nitrate, oxygen could serve as a terminal electron acceptor. Among many carbon sources tested, only a few sugars, fatty acids, and thiophene-2-carboxylate supported growth. Other heterocyclic compounds were not used. The sulfur atom of thiophene-2-carboxylate was oxidized to thiosulfate when cells were grown aerobically, or to elemental sulfur when cells were grown anaerobically with nitrate. Nitrate was reduced to nitrite. Growth on thiophene-2-carboxylate was dependent on the addition of molybdate to the medium. Tungstate, a specific antagonist of molybdate, inhibited growth on thiophene-2-carboxylate at concentrations 〉 10–7 M. Three inducible enzymes involved in the metabolism of thiophene-2-carboxylate were detected: an ATP-, CoA-, thiophene-2-carboxylate- and Mg2+-dependent thiophene-2-carboxyl-CoA ligase (AMP-forming), a molybdenum-containing thiophene-2-carboxyl-CoA dehydrogenase, and a thiophene-2-carboxyl-CoA thioesterase. The sequence of the 16S rRNA gene suggested a classification of strain NKK within the α-subgroup of the Proteobacteria as a new genus and species, Aquamicrobium defluvii gen. nov. sp. nov. (DSM 11603), closely related to Mesorhizobium sp. and Phyllobacterium sp., but representing a distinct lineage equal in depth to those of the two mentioned genera. Aquamicrobium defluvii can be distinguished from both genera by a distinct spectrum of substrates, the maximal growth temperature, and a different salt tolerance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050575
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  • 4
    Electronic Resource
    Electronic Resource
    The phylogenetic structure of the genus Acinetobacter (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 124 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract 16S rDNA sequence analysis was performed on the type strains of all validly described Acinetobacter species and five unnamed Acinetobacter strains. The phylogenetic analyses confirm that Acinetobacter is a coherent genus within the gamma subclass of Proteobacteria and that the species are phylogenetically well defined. A. calcoaceticus, A. lwoffii, A. johnsonii and A. haemolyticus form one cluster of closely related species, the pair A. junii and A. baumannii forms a second cluster. A. radioresistens stands phylogenetically isolated. The study reveals that three undescribed strains can be assigned to individually described species, while strains DSM 30009 and DSM 590 may represent two novel Acinetobacter species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb07307.x
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  • 5
    Electronic Resource
    Electronic Resource
    16S rDNA studies on members of Arthrobacter and Micrococcus: An aid for their future taxonomic restructing (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 123 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract 16S rDNA sequence data was obtained for 11 species of Arthrobacter and 4 species of Micrococcus and compared with that from other members of the arthrobacterial lineage within the order Actinomycetales. The intermixing of members of these two genera and the placement of Renibacterium salmoninarum within the radiation of these two genera, as previously suggested by 16S rRNA cataloguing, is confirmed. The branching pattern reveals several closely related organisms that cluster around the type species of Arthrobacter and Micrococcus; these species are considered ‘core organisms’. A few species, however, branch outside the radiation of core organisms; these include Micrococcus kristinae, Micrococcus halophilus, and, as previously indicated, Micrococcus sedentarius and M. nishinomiyaensis. As phenotypic data that would support the exclusion of these four species from the genus Micrococcus are still lacking taxonomic conclusions should await more thorough comparative studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb07217.x
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  • 6
    Electronic Resource
    Electronic Resource
    The phylogenetic diversity of thermophilic members of the genus Bacillus as revealed by 16S rDNA analysis (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 115 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Sixteen thermophilic strains of the genus Bacillus, representing eight validly described and six invalidly described species, as well as one unassigned strain, were investigated by comparative 16S rDNA analyses and the sequences compared to the existing database for the genera Bacillus and Alicyclobacillus. The majority of strains were found to cluster in two groups represented by B. stearothermophilus and B. pallidus. Bacillus smithii, B. thermocloacae, and B. thermoruber are phylogenetically well separated and cluster within the radiation of mesophilic bacilli. The as yet undescribed taxon ‘B. flavothermus’ warrants species status. B. schlegelii and B. tusciae group peripherally with members of Alicyclobacillus and may be reclassified when more phenotypic data support their phylogenetic position.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb06639.x
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  • 7
    Electronic Resource
    Electronic Resource
    Phylogenetic evidence for the classification of Acidothermus cellulolyticus into the subphylum of actinomycetes (1993)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 108 (1993), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The primary structure of the 16S rDNA of Acidothermus cellulolyticus, a cellulolytic, asporogenous rod isolated from a hot spring, was determined by automated sequence analysis. This Gram-variable organism is not related to members of other aerobic thermophilic genera found in similar environments, such as Thermus and Thermomicrobium, but clusters within the phylogenetically defined radiation of the actinomycetes where it represents an individual subline of descent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1993.tb06068.x
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  • 8
    Electronic Resource
    Electronic Resource
    Truepera radiovictrix gen. nov., sp. nov., a new radiation resistant species and the proposal of Trueperaceae fam. nov. (2005)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 247 (2005), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Two isolates, belonging to a new species of a novel genus of the Phylum “Deinococcus/Thermus”, were recovered from hot spring runoffs on the Island of São Miguel in the Azores. Strains RQ-24T and TU-8 are the first cultured representatives of a distinct phylogenetic lineage within this phylum. These strains form orange/red colonies, spherical-shaped cells, have an optimum growth temperature of about 50°C, an optimum pH for growth between about 7.5 and 9.5, and do not grow at pH below 6.5 or above pH 11.2. These organisms grow in complex media without added NaCl, but have a maximum growth rate in media with 1.0% NaCl and grow in media containing up to 6.0% NaCl. The organisms are extremely ionizing radiation resistant; 60% of the cells survive 5.0 kGy. These strains are chemoorganotrophic and aerobic; do not grow in Thermus medium under anaerobic conditions with or without nitrate as electron acceptor and glucose as a source of carbon and energy, but ferment glucose to d-lactate without formation of gas. The organisms assimilate a large variety of sugars, organic acids and amino acids. Fatty acids are predominantly iso- and anteiso-branched; long chain 1,2 diols were also found in low relative proportions; menaquinone 8 (MK-8) is the primary respiratory quinone. Peptidoglycan was not detected. Based on 16S rRNA gene sequence analysis, physiological, biochemical and chemical analysis we describe a new species of one novel genus represented by strain RQ-24T (CIP 108686T= LMG 22925T= DSM 17093T) for which we propose the name Truepera radiovictrix. We also propose the family Trueperaceae fam. nov. to accommodate this new genus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsle.2005.05.002
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  • 9
    Electronic Resource
    Electronic Resource
    Anaerobic oxidation of hydrocarbons in crude oil by new types of sulphate-reducing bacteria (1994)
    [s.l.] : Nature Publishing Group
    Nature 372 (1994), S. 455-458 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Enrichment of hydrocarbon-oxidizing sulphate-reducing bac-teria on crude oil was attempted in strictly anoxic artificial medium with the same concentration of sulphate (28 mM), chloride, sodium, potassium, magnesium and calcium ions as natural sea water9. Inocula were from Guaymas ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/372455a0
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  • 10
    Electronic Resource
    Electronic Resource
    A novel thermostable nitrile hydratase (1998)
    Springer
    Extremophiles 2 (1998), S. 347-357 
    Details
    ISSN: 1433-4909
    Keywords: Key words Thermophile ; Bacillus ; Nitrile hydratase ; Thermostable
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel, nitrile-degrading, thermophilic microorganism belonging to the genus Bacillus and most closely related to strain DSM 2349 has been isolated. The strain grew optimally at 65°C with the constitutive expression of a thermostable intracellular nitrile hydratase. No aromatic-specific "benzonitrilase" activity was detected under any conditions. The enzyme, an α2β2 heterotetramer with a native molecular weight of 110 kDa, was purified to homogeneity. N-terminal sequence data showed no homology to known bacterial α subunit sequences but had a high level of identity with other bacterial N-terminal β subunit sequences. The purified enzyme had a broad pH-activity range (50% activity limits were pH 5.1 and 8.7) and was stable in aqueous solution up to 60°C in the absence of either substrates or substrate analogues. Substrate specificity was restricted to aliphatic nitriles, but an unusual preference for branched and cyclic aliphatic nitriles was noted. Turnover rates under optimum reaction conditions were 746 and 4580 sec−1 for acetonitrile and valeronitrile, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007920050078
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