Publication Date:
1998-02-28
Description:
The calcium-calmodulin-dependent kinase II (CaMKII) is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, CaMKII can undergo autophosphorylation, resulting in CaM-independent activity. A point mutation was introduced into the alphaCaMKII gene that blocked the autophosphorylation of threonine at position 286 (Thr286) of this kinase without affecting its CaM-dependent activity. The mutant mice had no N-methyl-D-aspartate receptor-dependent LTP in the hippocampal CA1 area and showed no spatial learning in the Morris water maze. Thus, the autophosphorylation of alphaCaMKII at Thr286 appears to be required for LTP and learning.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Giese, K P -- Fedorov, N B -- Filipkowski, R K -- Silva, A J -- AG13622/AG/NIA NIH HHS/ -- New York, N.Y. -- Science. 1998 Feb 6;279(5352):870-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9452388" target="_blank"〉PubMed〈/a〉
Keywords:
2-Amino-5-phosphonovalerate/pharmacology
;
6-Cyano-7-nitroquinoxaline-2,3-dione/pharmacology
;
Animals
;
Calcium-Calmodulin-Dependent Protein Kinase Type 2
;
Calcium-Calmodulin-Dependent Protein Kinases/genetics/*metabolism
;
Calmodulin/metabolism
;
Gene Targeting
;
Hippocampus/metabolism/*physiology
;
*Long-Term Potentiation/drug effects
;
*Maze Learning
;
Mice
;
Mice, Inbred C57BL
;
Mice, Mutant Strains
;
Patch-Clamp Techniques
;
Phosphorylation
;
Phosphothreonine/metabolism
;
Picrotoxin/pharmacology
;
Point Mutation
;
Pyramidal Cells/*physiology
;
Receptors, N-Methyl-D-Aspartate/physiology
;
Synaptic Transmission
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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