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  • 2
    ISSN: 0378-1119
    Keywords: Recombinant DNA ; Shine-Dalgarno sequence ; lipoprotein promoter ; mRNA secondary structure ; synonymous codon substitution ; translational efficiency
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Physics Letters A 178 (1993), S. 265-270 
    ISSN: 0375-9601
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0168-9002
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 20 (1985), S. 2023-2028 
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Phosphorus segregation to the free surface of an amorphous Fe44Ni36P14B6 alloy during annealing in the temperature range 180 to 300° C has been studied by Auger electron spectroscopy. The phosphorus concentration increased with annealing temperature with an abrupt change appearing at about 250° C. At 276° C the phosphorus Peak Height Ratio on the surface was three times as high as that of a sputtered surface. A step-heating scheme has been employed to observe the surface segregation of phosphorus in the temperature range 25 to 290° C. On heating, the segregation behaviour can be correlated with a degradation in toughness properties. It is concluded that the segregation of phosphorus onto the free surface of this amorphous alloy is an indication of some local atomic migration processes, for example clustering, occurring inside the bulk.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 259 (1998), S. 123-129 
    ISSN: 1617-4623
    Keywords: Key words RNA polymerase assembly ; Protein-protein contact ; Functional site mapping ; Yeast two-hybrid screening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract [Rpb1 and Rpb2] Mapping of the contact sites␣on two large subunits of the fission yeast Schizosaccharomyces pombe RNA polymerase II with two small subunits, Rpb3 and Rpb5, was carried out using the two-hybrid screening system in the budding yeast Saccharomyces cerevisiae. Rpb5 was found to interact with any fragment of Rpb1 that contained the region H, which is conserved among the subunit 1 homologues of all RNA polymerases, including the β' subunit of prokaryotic RNA polymerases. In agreement with the fact that Rpb5 is shared among all three forms of eukaryotic RNA polymerases, the region H of RNA polymerase I subunit 1 (Rpa190) was also found to interact with Rpb5. On the other hand, two-hybrid screening of Rpb2 fragments from RNA polymerase II indicated the presence of an Rpb3 contact site in the region H which is conserved among the subunit 2 homologues of all RNA polymerases, including the β subunit of prokaryotic RNA polymerases. Possible functions of the regions H in the subunits 1 and 2 are discussed.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 107 (1997), S. 169-174 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  A double staining method combining Ulex europaeus agglutinin I lectin (UEA-I) and collagen type IV staining was used to determine the capillary density and the number of capillaries relative to different fibre types in human skeletal muscles. The result of this combined staining was compared with that of other staining methods including amylase-periodic acid Schiff (PAS), UEA-I, anti-collagen type IV and anti-von Willebrand factor. Muscle biopsy specimens, 12 from M. vastus lateralis and 6 from M. soleus, were obtained from 18 healthy young men. Compared with amylase-PAS staining, double staining showed a larger number of capillaries surrounding type I (+9.6%), type IIA (+8.6%) and type IIB (+11.6%) fibres in the M. vastus lateralis specimens (P〈0.001 for all differences). The capillary to fibre ratio (cap·fibre–1) and the capillary density (cap·mm–2) were 8.3% (P〈0.002) and 7.9% (P〈0.001) larger, respectively. In the M. soleus specimens, cap·fibre–1 and cap·mm–2 were 7.4 and 9.9% larger, respectively, by double staining compared with PAS staining. Further comparisons showed that the cap·fibre–1 and cap·mm–2 obtained with double staining were similar to the values determined by the UEA-I staining, but greater than that measured by the collagen type IV method. The double staining gave a more marked stain of capillaries and revealed muscle fibre borders clearly, which is an advantage in studies that require comparisons between serial sections using computerised image analyses. It is concluded that the double staining method is superior to either the UEA-I, collagen type IV or the traditional amylase-PAS staining methods in analysing capillary density of normal human skeletal muscle.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of intelligent and robotic systems 4 (1991), S. 43-53 
    ISSN: 1573-0409
    Keywords: Robots ; control ; learning ; tracking control ; computed-torque control ; robustness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract This paper derives a learning control law to achieve trajectory following for a robot manipulator. The controller consists of two parts, a computed torque servo for the rigid body terms that can be modelled and a learning law for the unmodelled dynamics. An advantage of this method is that bounds can be assigned to the position and velocity tracking errors.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of intelligent and robotic systems 10 (1994), S. 173-190 
    ISSN: 1573-0409
    Keywords: Rigid-link robot manipulator ; DC actuator ; position and force tracking control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract This paper introduces a framework for the design of tracking controllers for rigid-link electrically-driven (RLED) robot manipulators operating under constrained and unconstrained conditions. We present an intuitive nonlinear control strategy that can easily be reformulated for robots performing high precision tasks. The main emphasis is placed on the development of controllers that incorporate both motion in freespace and under constrained conditions. Another novelty is the combined treatment of force control and compensation for actuator dynamics. Based on models of the robot dynamics and environmental constraints, a reduced order dynamic model is obtained for the mechanical subsystem with respect to a set of constraint variables. A design procedure for tracking controllers is then formulated for the reduced order manipulator dynamics and the DC actuator dynamics. This paper concentrates on the theoretical aspects of the problem and, hence, is based on exact knowledge of the entire system. However, we have illustrated recently in [1] that this assumption can be generously relaxed in the design of a robust controller following a similar procedure as discussed in this paper.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Astrophysics and space science 208 (1993), S. 229-244 
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A simple method is proposed to infer the vector magnetic field at the surface of the Sun from Stokes profiles. This is based on the assumption that the variations of thermodynamical and magnetic field parameters with depth near the surface are so small that the displacements of the wavelengths at which the Stokes profiles reach their extrema can be ignored. And hence the polarized radiative transfer equations are greatly reduced to a set of non-linear equations with vector magnetic field parameters (Η, ϒ, χ) which can be solved by a numerical iteration method. By fitting the synthetic profiles, it is shown that this proposed method can produce information on the vector field at the surface. It is also used to revise the observed profiles and it is found that the observed sunspot has the magnetic field structure of the fan model with the lines of magnetic field twisted.
    Type of Medium: Electronic Resource
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