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Yeast activators stimulate plant gene expression (1988)

Ma, Jun ; Przibilla, Elisabeth ; Hu, Jim ; [et al.]

[s.l.] : Nature Publishing Group

Nature 334 (1988), S. 631-633

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Figure 1 shows the three types of DNA constructs used in this study. The first type, the 'effector', expresses various GAL4 derivatives from the nopaline synthase (NOS) promoter15. These derivatives include: GAL4(1-147), the ammo terminal portion of the 881-amino-acid GAL4 protein, which binds DNA ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/334631a0

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Development of chloroplast membranes during light induced greening ofChlorella fusca g-2 (1987)

Przibilla, Elisabeth ; Galling, G.

Springer

Protoplasma 139 (1987), S. 41-50

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ISSN: 1615-6102

Keywords: Chlorella fusca g-2 ; Freeze-fracture electron microscopy ; Thylakoid biogenesis

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary The development of thylakoid protein complexes during light induced greening of a mutant ofChlorella fusca was studied. Separation of chlorophyll-protein complexes and thylakoid polypeptides by LDS-polyacrylamide gel electrophoresis show that cells grown in the dark contain proteins belonging to coupling factor and cytochrome f/b6 complex. Parts of both reaction centers are present also. The antennae complexes are specifically lost in yellow cells. The changes in polypeptide pattern at different stages of development in the light are related to ultrastructural changes. The beginning of membrane appression can be correlated with the appearance of the light-harvesting complex II. While the average diameter of EF-particles increases throughout the greening process, their densitiesapart from the rearrangement due to membrane stacking-remain fairly constant. The kinetics of EFu-particle enlargement are different from those of EFs-particles. PF-faces in thylakoids grown in the dark contain particles of uniform diameter but some of them protrude more from the fracture plane than do their neighbors. During the first hours of greening, their density increases and two classes develop. From the beginning of membrane stacking, the composition of PFu-faces remains constant and PFs-particles increase in number for some time. Results are discussed on the basis of present knowledge of structurefunction relations in thylakoids.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01417534

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