ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
SUMMARY— The level of phosphoglucomutase in potato tubers has been found to be about 400 times higher than previously reported. This enzyme has been purified 40-fold over the dialyzed crude extract by a procedure consisting of fractionation with ethanol and acetone, fractionation with ammonium sulfate, and filtration through Sephadex G-100. The potato enzyme is similar to phosphoglucomutases obtained from other sources, but most closely resembles the rabbit muscle enzyme. It requires Mg2+ and cysteine for activity, is activated by preincubation with Mg2+ and cysteine, possesses a molecular weight of 63,000, exhibits optimal activity at pH 7.6, and is inhibited by anions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1967.tb09690.x
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