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  • 1
    Electronic Resource
    Electronic Resource
    Screening and characterization of bioflocculant produced by isolated Klebsiella sp. (1999)
    Springer
    Applied microbiology and biotechnology 52 (1999), S. 698-703 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Sixteen strains of polymer-producing bacteria were isolated from the activated sludge samples taken from two seafood processing plants in Southern Thailand. Their culture broths possessed the ability to flocculate kaolin suspension in the presence of 1% CaCl2. Based on the flocculating activity, the strain S11 was selected and identified to be a Klebsiella sp. using the partial 16S rRNA sequencing method. The growth of the isolated Klebsiella sp. was maximal (1.026 g l−1 dry cell mass) after 1 day cultivation while the highest polymer yield (0.973 g l−1) was achieved after 5 days cultivation. The flocculating activity of the culture broth, however, was highest after 2 days cultivation. The polymer was identified to be an acidic polysaccharide containing neutral sugar and uronic acid as its major and minor components, respectively. Results on the properties of the partially purified polysaccharide from Klebsiella sp. S11 revealed that it consisted of galactose, glucose and mannose in an approximate ratio of 5:2:1. It was soluble in acidic or basic solutions but not in organic solvents. Its molecular mass was greater than 2 × 106 Da. Infrared spectra showed the presence of hydroxyl, carboxyl and methoxyl groups in its molecules. Differential scanning calorimetry of the polysaccharide indicated the crystalline melting point (T m) at 314 °C. The optimum dosage of polysaccharide to give the highest flocculating activity was 15 mg l−1 in the presence of 1% CaCl2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051581
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and selection of cellulolytic fungi from palm oil mill effluent (1992)
    Springer
    World journal of microbiology and biotechnology 8 (1992), S. 614-617 
    Details
    ISSN: 1573-0972
    Keywords: Cellulolytic fungi ; isolation ; medium optimization ; palm oil mill effluent ; selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Cellulolytic fungi, 34 strains, were isolated from samples taken from palm oil mill residues and effluent, and high cellulase producers selected in comparison with nine known reference strains. Although 13 isolates showed good filter paper distintegration within 14 days, only eight isolates exhibited clearing zones around their colonies on carboxymethylcellulose (CMC) agar medium. Quantitative cellulase activity measurements, using CMC as carbon source, selected three of the eight isolates as potential cellulase producers. Using dried palm oil mill condensate as carbon source, only one of the isolates (F 11) showed similar results on both carbon sources. During media optimization for CMCase production, a four-fold increase from 0.058 to 0.275 U/ml was obtained using a medium, containing 0.1% (v/v) Tween 80 0.02% (w/v) NH4NO3, 0.025% (w/v) proteose-peptone and 0.1% (w/v) CMC dissolved in undiluted condensate from the sterilization of oil palm bunches, with an initial pH of 5.5.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01238799
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  • 3
    Electronic Resource
    Electronic Resource
    Production of cellulolytic enzymes from fungi and use in the saccharification of palm cake and palm fibre (1992)
    Springer
    World journal of microbiology and biotechnology 8 (1992), S. 536-538 
    Details
    ISSN: 1573-0972
    Keywords: Cellulases ; cellulose ; fungi ; palm cake ; palm fibre ; saccharification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Aspergillus niger ATCC 6275 possesses the highest carboxymethyl-cellulase, xylanase and β-glucosidase activities under liquid and solid cultivations compared withMyceliophthora thermophila IFO 31843 and an isolate, F11. Palm cake proved to be a better substrate for enzyme production and saccharification than palm fibre. Saccharification of these two substrates, using crude enzyme solutions from three fungi and commercial enzymes, was investigated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01201957
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization of proteases produced by newly isolated and identified proteolytic microorganisms from fermented fish (Budu) (1992)
    Springer
    World journal of microbiology and biotechnology 8 (1992), S. 284-286 
    Details
    ISSN: 1573-0972
    Keywords: Bacillus ; budu ; fermentation ; fish ; proteases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Eight different strains ofBacillus were isolated from fermented fish (Budu) and their proteolytic enzyme activities were determined after 18 h cultivation at room temperature (35° C). Four isolates possessed high protease activities. Optimum pH for these enzymes was between 7.0 and 8.0 and the optimal temperature was 55° C. The proteases retained 40% of their original activity after 20 min at 55° C but lost all activity at 65° C. Three of the four isolates were identified asBacillus subtilis, the fourth asBacillus licheniformis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01201880
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  • 5
    Electronic Resource
    Electronic Resource
    Optimization for growth of Rhodocyclus gelatinosus in seafood processing effluents (1993)
    Springer
    World journal of microbiology and biotechnology 9 (1993), S. 593-596 
    Details
    ISSN: 1573-0972
    Keywords: Effluent ; optimization ; Rhodocyclus ; tuna condensate ; waste utilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Tuna condensate was a better substrate than shrimp-blanching water or effluent from a frozen-seafood plant for growing Rhodocyclus gelatinosus under anaerobic conditions in the light. One strain out of four examined, R7, gave the highest biomass (4.0 g/l), cell yield (0.32 g cell/g COD), and COD removal (78%) in 1:10 (v/v) diluted tuna condensate. Shrimp-blanching water added to the tuna condensate further increased growth rate, biomass and COD removal. Optimal growth was at pH 7.0 and 3000 Lux light intensity. Acetate, pyruvate, glucose, glutamate, propionate or malate added to the tuna condensate did not increase cell yield, carotenoid or bacteriochlorophyll content or biomass protein. A maximum cell mass of 5.6 g/l (containing 50% protein) and 86% COD removal were obtained after 5 days' incubation under optimal conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386302
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  • 6
    Electronic Resource
    Electronic Resource
    Isolation, identification and growth conditions of photosynthetic bacteria found in seafood processing wastewater (1993)
    Springer
    World journal of microbiology and biotechnology 9 (1993), S. 590-592 
    Details
    ISSN: 1573-0972
    Keywords: Growth conditions ; isolation ; photosynthetic bacteria ; Rhodocyclus ; wastewater
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Four photosynthetic bacteria, isolated from 14 samples taken from seafood processing plants, were identified as species of Rhodocyclus gelatinosus, belonging to the purple, non-sulphur bacteria of the family Rhodospirillaceae. Cultivation in synthetic medium under four different conditions indicated that all four strains gave maximum carotenoid and bacteriochlorophyll synthesis under anaerobic conditions in the light, with values of 11 to 12.6 and 102 to 108 mg/g dry cell wt, respectively. These values are 87% higher than the pigment content obtained from aerobic cultivation, although the cell biomass of all strains (1.7 to 2.3 g/l) was 22 to 38% higher under aerobic conditions. Protein content was always between 32 and 43%. The specific growth rates of all isolates in aerobic cultivation (0.04 to 0.06 h-1) were twice those in anaerobic conditions in the light. No growth occurred in anaerobic conditions in the dark.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386301
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  • 7
    Electronic Resource
    Electronic Resource
    Anaerobic filter treatment of fishery wastewater (1994)
    Springer
    World journal of microbiology and biotechnology 10 (1994), S. 11-13 
    Details
    ISSN: 1573-0972
    Keywords: Anaerobic digestion ; biogas ; fishery waste ; wastewater
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Anaerobic treatment of wastewater from a selected seafood processing plant was conducted at organic loading rates (OLR) ranging from 0.3 to 1.8 kg chemical oxygen demand (COD)/m3.day and hydraulic retention times (HRT) ranging from 36 to 6 days. COD reduction decreased with increasing OLR. More than 75% COD reduction could be maintained up to an OLR of about 1 kg COD/m3.day with an HRT of 11 days. An OLR of 1.3 kg COD/m3.day corresponding to an HRT of 6.6 days gave maximal biogas productivity of 1.5 m3/m3.day or 1.3 m3 biogas/kg COD with a 65% COD reduction. If the HRT was kept constant at 11 days, an OLR of 1.3 kg COD/m3.day achieved maximal biogas productivity (1.1 m3/m3.day) and yield (0.75 m3/kg COD) and a 60% COD reduction for treatment of tuna condensate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00357553
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  • 8
    Electronic Resource
    Electronic Resource
    Optimization for xylanase and cellulase production from Aspergillus niger ATTC 6275 in palm oil mill wastes and its application (1997)
    Springer
    World journal of microbiology and biotechnology 13 (1997), S. 555-559 
    Details
    ISSN: 1573-0972
    Keywords: Aspergillus niger ; cellulase ; optimization ; palm oil wastes ; xylanase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Optimization of enzyme production from Aspergillus niger ATCC 6275 under both submerged and solid-substrate cultivation was investigated. Results from submerged cultivation using palm oil mill effluent revealed that pretreatment of ground palm cake did not improve enzyme production. Addition of 0.60g NH4NO3/l generated maximum activity of xylanase and cellulase (CMCase). The optimum aeration rate was 1.2 v/v min. Under solid-substrate cultivation, the results indicated that heating and alkali treatment of the ground palm cake gave no further improvement in enzyme production. The optimal N-source was 2% urea. Optimal initial moisture contents for xylanase and CMCase activities were 60% and 50% respectively, with temperature optima of 30°C and 35°C, respectively. The optimal inoculum size was 1× 108 spores/g palm cake with an initial pH of 4.5–5.0. The maximum activities of xylanase (282.9U/g) and CMCase (23.8U/g) were obtained under the optimum conditions. Solid-substrate cultivation was a better method for the production of enzyme, particularly xylanase, from A. niger ATCC 6275. The application of these enzymes to decanter effluent showed the separation of oil and grease and suspended solids from the effluent. This is comparable to the result achieved from using the commercial xylase preparation Meicelase and superior to the effect of Sumyzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018569426594
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  • 9
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    Screening and characterization of bioflocculant produced by isolated Klebsiella sp. (1999)
    Springer
    Details
    Publication Date: 1999-11-03
    Print ISSN: 0175-7598
    Electronic ISSN: 1432-0614
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Published by Springer
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  • 10
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    Optimization of Carbon Dioxide Reduction in Biohythane Using an Innovative Water Scrubber (2019)
    Institute of Physics
    Details
    Publication Date: 2019-04-09
    Print ISSN: 1757-8981
    Electronic ISSN: 1757-899X
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Published by Institute of Physics
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