ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

1

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The efficacy of repeated applications of the urease inhibitor N-(n-butyl) thiophosphoric triamide for improving the efficiency of urea fertilizer utilization on temperate grassland (1998)

Watson, C. J. ; Poland, P. ; Allen, M. B. D.

Oxford, UK : Blackwell Science Ltd

Grass and forage science 53 (1998), S. 0

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ISSN: 1365-2494

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: The effect of N-(n-butyl) thiophosphoric triamide (nBTPT)-amended urea on herbage dry-matter yield and nitrogen offtake by perennial ryegrass (Lolium perenne) was studied in fifteen grassland experiments at two sites in Northern Ireland between 1994 and 1996. The dry-matter yield and N offtake with applied urea was only significantly lower than that with applied calcium ammonium nitrate (CAN) on four occasions. On these occasions nBTPT increased the yield from the application of urea making it almost as effective as CAN. There was no evidence of any adverse effect on grass production with repeated applications of nBTPT-amended urea over a 3-year period and no indication that its efficacy to reduce NH3, loss from ureatreated swards declined when used repeatedly on the same soil.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1046/j.1365-2494.1998.5320137.x

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2

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Soil properties and the ability of the urease inhibitor N-(n-BUTYL) thiophosphoric triamide (nBTPT) to reduce ammonia volatilization from surface-applied urea

Watson, C.J. ; Miller, H. ; Poland, P. ; [et al.]

Amsterdam : Elsevier

Soil Biology and Biochemistry 26 (1994), S. 1165-1171

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ISSN: 0038-0717

Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1016/0038-0717(94)90139-2

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3

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Agronomic assessment and 15N recovery of urea amended with the urease inhibitor nBTPT (N-(n-butyl) thiophosphoric triamide) for temperate grassland (1994)

Watson, Catherine J. ; Poland, P. ; Miller, H. ; [et al.]

Springer

Plant and soil 161 (1994), S. 167-177

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ISSN: 1573-5036

Keywords: ammonia volatilization ; N-(n-butyl) thiophosphoric triamide ; 15N recovery ; temperate grassland ; urea ; urease inhibitor

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Abstract Three field experiments were undertaken concurrently at one site to evaluate a range of surface-applied nBTPT-amended urea products (0.01, 0.05, 0.1, 0.25 and 0.5% nBTPT w/w) on NH3 volatilization, grass yield and 15N recovery in the plant-soil system. Each experiment was repeated on five separate occasions over the 1992 growing season to cover a range of weather conditions. Total NH3 loss from unamended-urea ranged from 5.5% in early May to 20.8% in June. The inhibitor was highly effective in reducing ammonia volatilization and delaying the time at which maximum rate of NH3 loss occurred. Over all time periods the % inhibition was 50.4, 82.8, 89.0, 96.5 and 97.0% at the 0.01, 0.05, 0.1, 0.25 and 0.5% nBTPT levels respectively. There was no significant difference in the overall % inhibition in ammonia loss at different times suggesting that the effectiveness of the inhibitor was not dependent on climatic conditions. Over all times incorporation of nBTPT at the 0.05% level increased dry-matter yield by 9% compared to urea alone and increased the shoot recovery of N from 66.7% to 80.9%. Nitrogen saved from volatilization was taken up by the plant, however, the subsequent translation into dry-matter yield appeared to be adversely affected at the high inhibitor rates. There was no significant effect of inhibitor on 15N recovery in soil at any depth down to 15 cms. nBTPT significantly increased (p 〈 0.001) the % N derived from fertilizer (% N dff) in the shoot compared to unamended-urea and increased (p 〈 0.01) the shoot recovery of 15N from 32% up to 39%. Total 15N recovery in the soil-plant system was increased by up to 17% by amending urea with nBTPT. This urease inhibitor has been shown to improve the efficiency of urea however, its potential for the European market will be dependent on economic factors.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00046388

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4

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Differential expression of MUC1 on transfected cell lines influences its recognition by MUC1 specific T cells (1996)

Magarian-Blander, J. ; Hughey, R. P. ; Kinlough, C. ; [et al.]

Springer

Glycoconjugate journal 13 (1996), S. 749-756

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ISSN: 1573-4986

Keywords: MUC1 ; MHC-unrestricted ; tandem repeats ; tumour immunotherapy

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Abstract In adenocarcinomas of the breast and pancreas, underglycosylation of the glycoprotein MUC1, also expressed by normal breast and pancreatic ductal epithelial cells, results in new protein epitopes to which the immune system mounts a cytotoxic T cell response. This cytotoxic immune response is directed primarily against epitopes on the tandem repeat domain of MUC1, and is unconventional in that it is major histocompatibility complex (MHC)-unrestricted. It is therefore necessary to investigate the molecular basis of this immune response in order to enhance and optimize it for immune therapy purposes. In the present study, we characterize new MUC1 transfected human lymphoblastoid cell lines C1R and T2, and a pig kidney epithelial line LLC-PK1, that express MUC1 with either two repeats (MUC1–2R) or 22 repeats (MUC1–22R), and use them as stimulators and targets for cytotoxic T cells (CTL)in vitro. We show that MUC1–2R is processed and glycosylated similarly to MUC1–22R. In contrast to MUC1–22R, MUC1–2R is not recognized by CTL on T2 and C1R cells known for no or low MHC class I expression. It is however recognized when expressed at high density on xenogeneic LLC-PK1 cells. We propose that in MHC-unrestricted recognition, a large number of MUC1 epitopes is necessary to effectively engage the T cell receptor, and that in the presence of a low number of epitopes, engagement of the CD8 co-receptor by MHC class I molecules may be required for completing the signal through the T cell receptor.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00702339

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5

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Differential glycosylation of MUC1 in tumors and transfected epithelial and lymphoblastoid cell lines (1997)

Poland, P. A ; Kinlough, C. L ; Rokaw, M. D ; [et al.]

Springer

Glycoconjugate journal 14 (1997), S. 89-96

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ISSN: 1573-4986

Keywords: MUC1 ; mucin-like protein ; O-linked glycosylation ; apical targeting in epithelial cells

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Abstract The membrane-bound mucin-like protein MUC1 with a specified number of tandem repeats has been expressed by transfection of the cDNAs in both the epithelial cell lines MDCK and LLC-PK1, and human lymphoblastoid cell lines T2 and C1R. The structure and glycosylation states of the MUC1 in these four lines were compared with that of the endogenous MUC1 found in the human pancreatic (HPAF) and breast (BT-20) tumor cell lines using flow cytometry and Western blot analysis with anti-MUC1 antibodies, which are either sensitive or insensitive to the glycosylation state of the tandem repeat, and pretreatment of cells with phenyl-α-galactosaminide, an inhibitor of mucin sialylation. A similar analysis of MUC1 expression in transfected normal and O-glycosylation defective CHO cells reveals that the addition of galactose to the core oligosaccharide structure is apparently responsible for the anomalous difference in Mr between the mature and propeptide forms of the MUC1. Both the tumor cells and the transfected lymphoblastoid cells consistently express significant steady state levels of both the heavily glycosylated mature forms and the poorly glycosylated propeptide forms of the MUC1, whereas MUC1 is found predominantly as the mature extensively glycosylated species in the transfected epithelial cells. Immunofluoresence microscopy of cross sections of the polarized epithelial cells grown on culture filter inserts reveals that the MUC1 is clearly present at the apical surface of the cells, consistent with its expression in normal tissues. Thus, the successful expression of the MUC1 by transfection of either lymphoblastoid cells or epithelial cells yields model systems both for studying the natural structure/function relationships of the protein domains within the MUC1 molecule and for further elucidating the previously reported MHC-independent T-cell recognition of the MUC1.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1018569100438

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Spatiotemporal evolution of dike opening and décollement slip at Kīlauea Volcano, Hawai'i (2011)

E. K. Montgomery-Brown, D. K. Sinnett, K. M. Larson, M. P. Poland, P. Segall and A. Miklius

Wiley

In: Journal of Geophysical Research JGR - Solid Earth

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Publication Date: 2011-03-23

Description: Rapid changes in ground tilt and GPS positions on Kīlauea Volcano, Hawai'i, are interpreted as resulting from a shallow, two-segment dike intrusion into the east rift zone that began at 1217 UTC (0217 HST) on 17 June 2007 and lasted almost 3 days. As a result of the intrusion, a very small volume of basalt (about 1500 m3) erupted on 19 June. Northward tilt at a coastal tiltmeter, subsidence of south flank GPS sites, southeastward displacements at southwestern flank GPS sites, and a swarm of flank earthquakes suggest that a slow slip event occurred on the décollement beneath Kīlauea's south flank concurrent with the rift intrusion. We use 4 min GPS positions that include estimates of time-dependent tropospheric gradients and ground tilt data to study the spatial and temporal relationships between the two inferred shallow, steeply dipping dike segments extending from the surface to about 2 km depth and décollement slip at 8 km depth. We invert for the temporal evolution of distributed dike opening and décollement slip in independent inversions at each time step using a nonnegative least squares algorithm. On the basis of these inversions, the intrusion occurred in two stages that correspond spatially and temporally with concentrated rift zone seismicity. The dike opening began on the western of the two segments before jumping to the eastern segment, where the majority of opening accumulated. Dike opening preceded the start of décollement slip at an 84% confidence level; the latter is indicated by the onset of northward tilt of a coastal tiltmeter. Displacements at southwest flank GPS sites began about 18 h later and are interpreted as resulting from slow slip on the southwestern flank. Additional constraints on the evolution of the intrusion and décollement slip come from inversion of an Envisat interferogram that spans the intrusion until 0822 UTC on 18 June 2007, combined with GPS and tilt data. This inversion shows that up to 0822 UTC on 18 June, décollement slip is only required in a limited region offshore of Ka'ena Point. A similar inversion of the complete event, which includes GPS and tilt data up to 21 June and a second Envisat interferogram spanning the complete intrusion until 21 June, shows décollement slip spread westward across the south flank. This may suggest westward migration of the décollement slip as the event progressed.

Print ISSN: 0148-0227

Topics: Geosciences , Physics

Published by Wiley on behalf of American Geophysical Union (AGU).

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Evidence for core 2 to core 1 O-glycan remodeling during the recycling of MUC1 (2013)

Razawi, H., Kinlough, C. L., Staubach, S., Poland, P. A., Rbaibi, Y., Weisz, O. A., Hughey, R. P., Hanisch, F.-G.

Oxford University Press

In: Glycobiology

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Publication Date: 2013-06-29

Description: The apical transmembrane glycoprotein MUC1 is endocytosed to recycle through the trans -Golgi network (TGN) or Golgi complex to the plasma membrane. We followed the hypothesis that not only the known follow-up sialylation of MUC1 in the TGN is associated with this process, but also a remodeling of O -glycan core structures, which would explain the previously described differential core 2- vs core 1-based O-glycosylation of secreted, single Golgi passage and recycling membrane MUC1 isoforms (Engelmann K, Kinlough CL, Müller S, Razawi H, Baldus SE, Hughey RP, Hanisch F-G. 2005. Glycobiology. 15:1111–1124). Transmembrane and secreted MUC1 probes show trafficking-dependent changes in O-glycan core profiles. To address this novel observation, we used recombinant epitope-tagged MUC1 (MUC1-M) and mutant forms with abrogated clathrin-mediated endocytosis (MUC1-M-Y20,60N) or blocked recycling (palmitoylation-defective MUC1-M-CQC/AQA). We show that the CQC/AQA mutant transits the TGN at significantly lower levels, concomitant with a strongly reduced shedding from the plasma membrane and its accumulation in endosomal compartments. Intriguingly, the O-glycosylation of the shed MUC1 ectodomain subunit changes from preponderant sialylated core 1 (MUC1-M) to core 2 glycans on the non-recycling CQC/AQA mutant. The O-glycoprofile of the non-recycling CQC/AQA mutant resembles the core 2 glycoprofile on a secretory MUC1 probe that transits the Golgi complex only once. In contrast, the MUC1-M-Y20,60N mutant recycles via flotillin-dependent pathways and shows the wild-type phenotype with dominant core 1 expression. Differential radiolabeling of protein with [ 35 S]Met/Cys or glycans with [ 3 H]GlcNH 2 in pulse-chase experiments of surface biotinylated MUC1 revealed a significantly shorter half-life of [ 3 H]MUC1 when compared with [ 35 S]MUC1, whereas the same ratio for the CQC/AQA mutant was close to one. This finding further supports the novel possibility of a recycling-associated O -glycan processing from Gal1-4GlcNAc1-6(Gal1-3)GalNAc (core 2) to Gal1-3GalNAc (core 1).

Print ISSN: 0959-6658

Electronic ISSN: 1460-2423

Topics: Biology , Medicine