ISSN:
1573-4986
Keywords:
neoglycolipids
;
sialyltransferases
;
rat liver Golgi vesicles
;
lactosylceramide
;
GM3
;
GD3
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The cholesterol-containing lactose derived neoglycolipids β-Lactosylcholesterol, Cholesteryl-β-lactosylpropane-1,3-diol, 3-Cholesteryl-1-β-lactosylglycerol, 2-Cholesteryl-1-β-lactosylglycerol, 2,3-Dicholesteryl-1-β-lactosylglycerol, 1-Deoxy-1-cholesterylethanolaminolactitol, 1-Deoxy-1-cholesteryl (N-acetyl)-ethanolaminolactitol, 1-Deoxy-1-cholesterylphosphoethanolaminolactitol, and 1-Deoxy-1-cholesterylphospho (N-acetyl)-ethanolaminolactitol were synthesized and used as acceptors for sialytransferases from rat liver Golgi vesicles. Relative activities with the neoglycolipids as acceptors varied from 28 to 163% compared to those obtained with the authentic acceptor lactosylceramide. Product identification by thin layer chromatography and fast atom bombardment mass spectrometry showed that the neoglycolipids yielded mono- and disialylated products. The results of competition experiments suggested that lactosylceramide and the neoglycolipids were sialylated by the same enzymes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00731488
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