ISSN:
1432-072X
Keywords:
d-Alanine
;
l-Alanine
;
β-Alanine
;
Dehydrogenase
;
Oxidase
;
Pseudomonas aeruginosa
;
Catabolism
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Pseudomonas aeruginosa PA01 was found to utilise both thed- andl-isomers of α-alanine and also β-alanine as sole sources of carbon and energy for growth. Enzymological studies of wild-type cultures and comparison with mutants deficient in growth upon one or more isomers of alanine led to the following conclusions: (i) utilisation ofd-alanine involved its direct oxidation by an inducible, membrane-bound, cytochrome-linked dehydrogenase; (ii) utilisation ofl-alanine required its conversion to the directly oxidisabled-form by a soluble racemase; (iii) utilisation of β-alanine, likel-alanine, involves both the racemase andd-alanine dehydrogenase enzymes, but in addition must involve other enzymes the identity, of which is still speculative; (iv)P. aeruginosa, likeEscherichia coli, appears to take upd-alanine andl-alanine by means of two specific permeases.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00690240
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