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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Industrial and engineering chemistry 10 (1971), S. 548-551 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Industrial and engineering chemistry 11 (1972), S. 512-520 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of environmental contamination and toxicology 57 (1996), S. 236 -241 
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 17 (1975), S. 31-49 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A zinc sulfide concentrate was leached microbiologically by Thiobacillus ferrooxidans in a continuous stirred tank reactor. A model was developed to predict, the leaching kinetics when the bacterial growth rate was not limited by any substrate other than the zinc concentrate, and it was modified to explain the observed results.Stable steady sates were obtained over a range of dilution rates from 0.0171 to 0.1038 hr-1. Because a solid substrate was used, the specific growth rate of the bacaeria was not a unique function of the subastrate concentration, and conventional contnuous culture theory based on the Monod equation did not apply to this system. The leaching rates and bacterial growth rates were first order in mineral surface area cocentration.
    Additional Material: 7 Ill.
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 17 (1975), S. 939-939 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 41 (1993), S. 700-706 
    ISSN: 0006-3592
    Keywords: anaerobic digestion ; cheese whey ; UASB reactor ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The anaerobic digestion of cheese whey was studied in a UASB reactor. The profiles of the reactor, i.e., the distributions of the substrate concentration and pH under different operating conditions were developed. From the concentrations of substrates measured at various levels above the bottom of the reactor, two reaction stages, namely acidogenesis and methanogenesis, were distinguished. The instability caused by high influent concentration was interpreted as the accumulation of VFAs in the acidogenic stage beyond the assimilative capacity of the methanogenic stage. A range of stable operating conditions was predicted from the results of the profile measurements. The optimal influent concentration was found to be between 25 and 30 g COD/L at an HRT of 5 days for system stability. Other options fro stability control were discussed. © 1993 John Wiley & Sons, Inc.
    Additional Material: 7 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 41 (1993), S. 736-744 
    ISSN: 0006-3592
    Keywords: lactose ; effective diffusivity ; acidogenic biofilm ; biofilm void fraction ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Effective diffusivity of lactose in active acidogenic biofilms was measured at 35°C and pH 4.6 with a specially designed diffusion cell. The diffusion cell was designed and operated in such a way that the lactose concentrations on the surface and at the center of a living bacterial aggregate could be measured at steady state. As a model parameter in a widely accepted reaction-diffusion equation which describes lactose distribution in living biofilms, the effective diffusivity of lactose in the biofilms was found to be about 65% of the lactose diffusivity in free solutions. It was experimentally determined that the active biofilms had about 66% void volume made up of channels through which the lactose molecules were transported into the bacterial aggregates. Therefore, the decrease in lactose diffusivity was mainly caused by the biofilm's solid biomass fraction rather than the tortuosity of the channels. © 1993 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 45 (1995), S. 212-218 
    ISSN: 0006-3592
    Keywords: biofilms ; calcium ; anaerobic digestion ; acidogenesis ; lactose ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Anerobic biofilms with dominantly acidogenic bacteria were grown in fixed-bed recycle reactors. The influence of calcium concentration in the culture medium on biofilm mass accumulation, immobilized calcium concentration, and biofilm-specific activity was investigated. The results indicate that the biofilm mass accumulation was increased by the presence of calcium in the growth medium when calcium concentration was not higher than 120mg/L. Calcium accumulated in the biofilms increased in proportion to the calcium level in the feed. The biofilms for an increased input calcium concentration showed a trend of decrease in specific activity. The biofilms with a thickeness of less than 0.5 mm had the highest specific activity. The optimum calcium concentration for substrate consumption by the biofilms was 100 to 120 mg/L. The biofilms transferred from higher calcium medium to lower calcium medium were more susceptible to sloughing from their support surfaces, which indicates calcium's role in the stability of the biofilm structure. © 1995 John Wiley & Sons, Inc.
    Additional Material: 10 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 30 (1987), S. 88-95 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cheese whey is the main component of waste streams from cheese manufacturing plants. Whey is a high biochemical oxygen demand (BOD) effluent that must be reduced before the streams are sent to the sewer. It is proposed in this article that the production of methane by anaerobic fermentation would be the best use of this stream, especially for small plants. Single-stage fermentation of lactose, the main component of whey, results in a very low pH and a stalled process. Two-phase fermentation will eliminate this problem. The acidogenic stage of fermentation has been studied at pH of between 4 and 6.5. The nature of the main products of the reaction have been found to be pH dependent. Below a pH of 4.5 a gas (CO2 and H2) is produced along with ethanol, acetate, and butyrate. Above a pH of 4.5 no gas was produced, and the liquid products included less ethanol and butyrate and more acetate. A separate study on the conditions for gas formation showed that if the pH dropped for a short time below 4.5 gases were formed at all subsequent pH. This would indicate a change in population distribution due to the period at a low pH. By assuming that the desired products from the acidogenic stage were butyrate, acetate, and no gases, the optimum pH range was found to be between 6.0 and 6.5.
    Additional Material: 6 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 34 (1989), S. 1235-1250 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Acidogenic fermentation of lactose was carried out in a continuous stirred reactor with a mixed anaerobic culture. From the variation of the reactor products with pH and dilution rate two possible carbon flow schemes were proposed for the reaction. In both schemes the carbon flow from pyruvate to butyrate and lactate was assumed to occur in parallel. A change in gas composition and in product concentrations at dilution rates between 0.1 and 0.15 h-1 for pH levels between 4.5 and 6.0 was ascribed to a shift in microbial population. To clarify the mechanism radiotracer tests were made using [U-14C]-butyrate, [2-14C]-propionate and [U-14C]-lactate to determine the path of carbon flow during acidogenesis of lactose using a mixed culture. At a dilution rate between 0.1 and 0.15 h-1 and pH from 4.5 to 6.0 a rise in the lactate concentration in the product was shown to be due to a microbial population shift which disabled the conversion of lactate to other intermediary metabolites. It was also found that the flow of carbon from pyruvate to butyrate and lactate occurred by parallel pathways. Also, in the presence of hydrogen reducing methanogens, lactate was almost completely converted to acetate and not propionate. Butyrate was found to be converted to acetate at a slow rate as long as hydrogen reducing methanogens were present. The role played by propionibacteria in this lactose acidogenic eocosystem was minor. From the carbon flow model it can be concluded that lactate is the most suitable marker for optimizing an acidogenic reactor in a two-phase biomethanation process.
    Additional Material: 16 Ill.
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