ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
A sensitive secondary antibody system was developed for detection of small amounts of group specific component (Gc). For the first antibody step after focusig, a comparison between immunofixation on cellulose acetate membranes and capillary blotting on nitrocellulose was made. The sensitivity of the first is much better than that of the latter. The method allowed the detection of about 150 picograms of Gc in 1:12 800 diluted serum samples. Even in six-year-old badly soluble bloodstains kept at room temperature, Gc could be phenotyped. The 250 μm thick immobilized pH-gradient gels for focusing of Gc can be used repeatedly after washing and drying to the initial weight without destroying the pH gradient.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150070606
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