ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
The genetic variants of bovine β-lactoglobulin (β-lg) from the “Murnau-Werdenfelser” breed were analyzed in three different isoelectric focusing (IEF) systems. While carrier ampholyte IEF with a pH gradient of 0.2 pH/cm did not resolve the new variant W from the B variant and IEF in immobilized pH gradients (IPG) with 0.1 pH/cm only partially resolved it, adequate separation was achieved with IPG-IEF in a pH 5.25-pH 5.7 gradient, in presence of 0.8 % w/v carrier ampholytes, both over a 10 and 17 cm separation distance. Apparent isoelectric points (pI's) and genetic frequencies (f) were as follows: β-lg A, pI = 5.370,f = 0.364; β-lg B, pI = 5.485,f = 0.480; β-lg W, pI = 5.492,f = 0.076; and β-lg D, pI = 5.610,f = 0.080. The small difference of Δ pI = 0.007 between β-lg B and β-lg W respectively, seems to originate from a “silent” substitution of neutral amino acid residues as compared to the larger Δ pI's of the other genetic variants of β-lg, which result from substitution of charged amino acids.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150090925
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