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  • 1
    Electronic Resource
    Electronic Resource
    Preparation of a tRNA-dependent wheat germ protein-synthesizing system (1989)
    Springer
    Plant molecular biology 12 (1989), S. 301-306 
    Details
    ISSN: 1573-5028
    Keywords: protein ; synthesis ; tRNA-dependent ; wheat germ
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method is described for the preparation of a tRNA-dependent wheat germ protein-synthesizing system. This system can be supplied with exogenous tRNAs of eukaryotic or prokaryotic origin. In order to obtain maximal aminoacylation of the added tRNAs, the translation assays can be supplemented with homologous aminoacyl-tRNA synthetase preparations. Such a tRNA-dependent wheat germ protein-synthesizing system, which is easy to prepare, can be used not only to translate plant cytoplasmic mRNAs in the presence of added cytoplasmic tRNAs, but also to determine the translation activity of exogenous tRNAs from various sources in the presence of either natural or in vitro synthesized mRNAs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043207
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  • 2
    Electronic Resource
    Electronic Resource
    Codon recognition mechanisms in plant chloroplasts (1990)
    Springer
    Plant molecular biology 14 (1990), S. 805-814 
    Details
    ISSN: 1573-5028
    Keywords: chloroplast ; codon-anticodon interaction ; codon reading mechanism ; tRNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In chloroplasts, all 61 sense codons are found in chloroplast (cp) DNA sequences coding for proteins. However among the sequenced cp tRNAs or tRNA genes, tRNAs with anticodons complementary to codons CUU/C (Leu), CCU/C (Pro), GCU/C (Ala) and CGC/A/G (Arg) [or CGC/A (Arg) in Marchantia] have not been found. In this paper we show that cp tRNAAla(U*GC), cp tRNAPro(U*GG) and cp tRNAArg(ICG) are able to decode the corresponding four-codon family. In the case of leucine codons CUU/C, we show that ‘U:U and U:C wobble’ mechanisms can operate to allow the reading of these codons by cp tRNALeu (UAm7G).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016513
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  • 3
    Electronic Resource
    Electronic Resource
    Variations during leaf development of the relative amounts of two bean (Phaseolus vulgaris) chloroplast tRNAsPhe which differ in their minor nucleotide content (1990)
    Springer
    Plant molecular biology 14 (1990), S. 969-975 
    Details
    ISSN: 1573-5028
    Keywords: bean ; chloroplast tRNAPhe ; leaf development ; nucleotides (minor)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Bean (Phaseolus vulgaris cv. Saxa) chloroplasts contain two tRNAPhe species, namely tRNAPhe1 and tRNAPhe2. By sequence determination, we show that tRNAPhe2 is identical to the previously sequenced tRNAPhe1 except for two undermodified nucleotides. By reversed-phase chromatography analyses, we demonstrate that the relative amounts of these two chloroplast tRNAsPhe vary during leaf development: in etiolated leaves the undermodified tRNAPhe2 only represents 15% of total chloroplast tRNAPhe, during development and greening it increases to reach 60% in 8-day-old leaves, and it then decreases to 9% in senescing leaves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019393
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