ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary Bacillus thuringiensis var.israelensis protein toxin radiolabeled with14C-iodoacetamide was bound to latex beads. The efficiency of attachment was not affected by the temperature or length of incubation or by the presence of several different buffers, salts, or organic solvents. However, attachment decreased dramatically at pH values 〉8.5 or in the presence of detergents (anionic, neutral, or cationic). Protein concentrations ≥2–3 mg/m2 of bead surface led to a progressively decreasing efficiency of protein adsorption. With minor variations, these findings should also be applicable to the attachment of numerous other proteins of diagnostic significance.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01982555
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