ISSN:
1432-1955
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Spent culture medium from in vitro cultures ofPlasmodium falciparum was used as the source for immunoadsorbent enrichment of soluble parasite antigens. IgG obtained fromP. falciparum-hyperimmune Liberian serum was used as the ligand in the immunoadsorbent. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the immunoadsorbent isolated material revealed the presence of at least 15 antigenic and parasite-derived polypeptides. Immunoblotting after SDS-PAGE showed 〉20 antigenic polypeptides in the molecular weight range of 15–250 kDa. Several of these were heat-stable (100°C for 5 min), and six of them were detected even after heating for 60 min. Among the latter antigens was Pf155/RESA, a merozoite antigen deposited in the erythrocyte membrane at invasion. Pf155/RESA can be detected using a modified erythrocyte membrane immunofluorescence (EMIF) assay. Inhibition of EMIF with heated parasite material showed that the antigenic activity was intact after heating for up to 30 min. The immunofluorescence-inhibitory activity was separated into two fractions, one containing Pf155/RESA and one containing polypeptides with molecular weights of 135 and 120 kDa. All three of these antigenic polypeptides bound selectively, albeit nonspecifically, to aminoethyl-BioGel resins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00934380
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