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  • 1
    Monograph available for loan
    Monograph available for loan
    The last cruise of the CARNEGIE (1932)
    Baltimore : Williams & Wilkins
    Details Availability
    Call number: 1953 / Regal 12
    Type of Medium: Monograph available for loan
    Pages: XVII, 331 S. : Ill.
    Location: Magazine - must be ordered
    Branch Library: GFZ Library
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  • 2
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    The genome of deep-sea vent chemolithoautotroph Thiomicrospira crunogena XCL-2 (2007)
    Public Library of Science (PLoS)
    Details
    Publication Date: 2022-05-25
    Description: This is an open-access article distributed under the terms of the Creative Commons Public Domain dedication. The definitive version was published in PLoS Biology 4 (2006): e383, doi:10.1371/journal.pbio.0040383.
    Description: Presented here is the complete genome sequence of Thiomicrospira crunogena XCL-2, representative of ubiquitous chemolithoautotrophic sulfur-oxidizing bacteria isolated from deep-sea hydrothermal vents. This gammaproteobacterium has a single chromosome (2,427,734 base pairs), and its genome illustrates many of the adaptations that have enabled it to thrive at vents globally. It has 14 methyl-accepting chemotaxis protein genes, including four that may assist in positioning it in the redoxcline. A relative abundance of coding sequences (CDSs) encoding regulatory proteins likely control the expression of genes encoding carboxysomes, multiple dissolved inorganic nitrogen and phosphate transporters, as well as a phosphonate operon, which provide this species with a variety of options for acquiring these substrates from the environment. Thiom. crunogena XCL-2 is unusual among obligate sulfur-oxidizing bacteria in relying on the Sox system for the oxidation of reduced sulfur compounds. The genome has characteristics consistent with an obligately chemolithoautotrophic lifestyle, including few transporters predicted to have organic allocrits, and Calvin-Benson-Bassham cycle CDSs scattered throughout the genome.
    Description: This work was performed under the auspices of the United States Department of Energy by Lawrence Livermore National Laboratory, University of California, under contract W-7405-ENG-48. Genome closure was funded in part by a University of South Florida Innovative Teaching Grant (to KMS). KMS, SKF, and CAK gratefully acknowledge support from the United States Department of Agriculture Higher Education Challenge Grants Program (Award # 20053841115876). SMS kindly acknowledges support through a fellowship received from the Hanse Wissenschaftskolleg in Delmenhorst, Germany (http://www.h-w-k.de). MH was supported by a Woods Hole Oceanographic Institution postdoctoral scholarship.
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Format: 4436005 bytes
    Format: application/pdf
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  • 3
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    Growth performance and cost efficiency of tilapia (Oreochromis niloticus) and milkfish (Chanos chanos) fed extruded floating and non-floating feeds reared in net cages in Taal Lake (2021)
    In:  http://aquaticcommons.org/id/eprint/26761 | 25026 | 2021-02-27 20:59:44 | 26761 | National Fisheries Research and Development Institute, Philippines
    Details
    Publication Date: 2021-07-24
    Description: This study evaluated the growth and cost efficiency of tilapia (Oreochromis niloticus) and milkfish (Chanos chanos) fed three feed types used in cage farming in Taal Lake, Batangas, Philippines to serve as baseline information for cage aquaculture regulations. O. niloticus and C. chanos were reared in net cages fed three feed treatments: extruded floating feed (EFF), slow-sinking feed (SSF), and sinking feed (SF). Growth performance, feed conversion ratio (FCR), yield, and cost efficiency were compared at harvest. Results of the study showed that EFF had significantly higher mean weight gain, absolute growth and specific growth rate, biomass harvest, percentage good size fish, and FCR than SF in both O. niloticus and C. chanos (P〈0.05), but had no significant difference with SSF in terms of growth parameters (P〉0.05). FCR and biomass harvest were significantly higher in EFF than SF in O. niloticus (P〈0.05) but were not statistically different in C. chanos (P〉0.05). Survival rate was not significantly different among feed types (P〉0.05) in both species. Net profit was significantly higher in EFF than the other feed types (P〈0.05). At the same volume of fish production in the lake, the use of extruded floating feeds in cages lessened the feed cost by 17.91-29.44% for higher net returns and decreased feeds use by 19.64-30.0%, which could minimize negative impacts on the lake water environment. The results of the study revealed the comparative advantage of floating feeds over slow-sinking feeds and sinking feeds and is therefore recommended as the ideal feed type for cage farming in the lake.
    Keywords: Aquaculture ; Fisheries ; extruded floating feeds ; slow sinking feeds ; sinking feeds ; growth performance ; cage aquaculture
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 41-56
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  • 4
    Electronic Resource
    Electronic Resource
    Detection of exogenous gene sequences in dissolved DNA from aquatic environments (1989)
    Springer
    Microbial ecology 18 (1989), S. 21-28 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method for the concentration and detection of gene sequences in the dissolved DNA from freshwater and marine environments has been developed. The limit of detection in the dot blot format was 167 fg/ml (100 ml sample) for exogenous herpes simplex thymidine kinase (TK) gene that was added to artificial seawater or river water. This procedure has been used to determine the longevity and monitor progressive changes in molecular weight of a plasmid containing the TK gene added to eutrophic estuarine water. The onset of plasmid degradation as determined by change in molecular weight was rapid (within 5 min). Intact plasmid was detected for at least 4 hours and sequences hybridizable to the TK gene probe were present for up to 24 hours.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02011693
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Natural transformation of a marineVibrio species by plasmid DNA (1990)
    Springer
    Microbial ecology 19 (1990), S. 259-268 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Vibrio sp. DI9, recently isolated from Tampa Bay, FL, has been found to be naturally transformed by the broad host range plasmid pKT230 in both filter transformation assays and sterile sediment microcosms. This is the first report of natural transformation by plasmid DNA of aVibrio sp. and of a marine bacterial isolate. Transformation frequencies ranged from 0.3 to 3.1×10−8 transformants per recipient. Transformants were detected by both plating and by selection for growth in liquid medium in the presence of streptomycin and kanamycin and confirmed by probing of southern transfers. Transformation was enhanced by multimeric forms of the plasmid. A technique using sediment microcosms, mixed populations ofVibrio sp. DI9 and another antibiotic resistant organism, and enrichment in liquid media has been developed which allows detection of transformation at frequencies too low to be detected by plating. This technique may serve as a model for the detection of natural transformation in the environment. These results suggest that natural transformation may be one mechanism of horizontal plasmid transfer in the marine environment, and may provide the methodology with which to detect this process in natural populations of bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02017170
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  • 6
    Electronic Resource
    Electronic Resource
    Plasmid transfer to indigenous marine bacterial populations by natural transformation (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 15 (1994), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Horizontal gene transfer among microbial populations has been assumed to occur in the environment, yet direct observations of this phenomenon are rare or limited to observations where the mechanism(s) could not be explicitly determined. Here we demonstrate the transfer of exogenous plasmid DNA to members of indigenous marine bacterial populations by natural transformation, the first report of this process for any natural microbial community. Ten percent of marine bacterial isolates examined were transformed by plasmid DNA while 14% were transformed by chromosomal DNA. Transformation of mixed marine microbial assemblages was observed in 5 of 14 experiments. In every case, acquisition of the plasmid by members of the indigenous flora was accompanied by modification (probably from genetic rearrangement or methylation) that altered its restriction enzyme digestion pattern. Estimation of transformation rates in estuarine environments based upon the distribution of competency and transformation frequencies in isolates and mixed populations ranged from 5 × 10−4 to 1.5 transformants/1 day. Extrapolation of these rates to ecosystem scales suggests that natural transformation may be an important mechanism for plasmid transfer among marine bacterial communities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1994.tb00237.x
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  • 7
    Electronic Resource
    Electronic Resource
    Diel Patterns of Regulation of rbcL Transcription in a Cyanobacterium and a Prymnesiophyte (2000)
    Springer
    Marine biotechnology 2 (2000), S. 429-436 
    Details
    ISSN: 1436-2236
    Keywords: Key words: diel patterns, transcription, rbcL, cyanobacterium, prymnesiophyte.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract: Diel patterns of rbcL transcription, ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) activity, and whole cell carbon fixation were compared in the marine cyanobacterium Synechococcus PCC7002 and the chromophytic prymnesiophyte Pavlova gyrans. Both organisms were grown on a 12:12 light-dark cycle, with the light period starting at 0700. Strong diel patterns in these three aspects of carbon fixation occurred in both organisms, with maximal levels in the light period and minima in the dark. In Synechococcus, maximal rbcL transcript abundance occurred at noon and was followed by rapid disappearance. RubisCO enzyme activity and whole cell carbon fixation were elevated at 1600, and they disappeared over the next 8 hours. In contrast, in Pavlova, rbcL transcript abundance was maximal at 1600, and it was maintained at 66% of this level into the dark period (2000). Whole cell carbon fixation and RubisCO activity were elevated into the dark period (at 2000), being 77% and 81%, respectively, of the maximum. A similar diel pattern of cyanobacterial-like and chromophyte-like rbcL transcription has been observed in natural phytoplankton populations. These studies suggest that chromophytes are more adapted to take advantage of carbon fixation late in the day than cyanophytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101260000016
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  • 8
    Electronic Resource
    Electronic Resource
    The distribution of dissolved DNA in an oligotrophic and a eutrophic river of Southwest Florida (1991)
    Springer
    Hydrobiologia 218 (1991), S. 53-63 
    Details
    ISSN: 1573-5117
    Keywords: dissolved DNA ; oligotrophic and eutrophic rivers ; dissolved organic carbon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The distribution of dissolved DNA concentrations and some microbial variables were compared in an oligo-mesotrophic river (the Crystal River) and a phosphate-rich eutrophic river (the Alafia River) in Southwest Florida over a 15 month period. Concentrations of phosphate and nitrate in the Alafia River averaged 135 and 18.2 times the respective phosphate and nitrate concentrations of the oligo-mesotrophic Crystal River. The seasonal average dissolved DNA concentration for the Alafia River exceeded that of the Crystal River by a factor of 1.8 (8.2 μg 1−1 compared to 4.6 μg 1−1, respectively). The greatest concentrations of dissolved DNA in the Alafia River were found in areas that contained the largest populations of phytoplankton and bacteria (a reservoir formed from an abandoned phosphate mining pit and two downstream stations near the mouth of the river). Differences in dissolved DNA concentrations between these environments and more pristine environments (i.e. all Crystal River Stations and upstream Alafia River stations) were of the same order of magnitude (1.8 to 2.2-fold) as the differences in bacterial abundance and activity, but considerably less than differences in phytoplankton abundance and activity between such environments. Seasonal variations in dissolved DNA concentrations in the Crystal River corresponded to seasonal variations in microbial populations, with minimal values in January and greater values in July. In the Alafia River, lowest concentrations for dissolved DNA occurred in July during the wet season, when seasonal flooding of area of leaf litter yielded high levels of dissolved organic carbon (DOC) which were low in dissolved DNA. These results suggest that: 1) in situ planktonic activity is a greater source of dissolved DNA than allochthonous or terrestrial sources of DOC; 2) factors that control the magnitude of heterotrophic bacterial populations are more likely to control dissolved DNA levels than factors regulating autotrophic population activity and abundance; 3) differences in dissolved DNA between eutrophic and oligo-mesotrophic environments are often much smaller than the differences in nutrient concentration between such environments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00006418
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  • 9
    Electronic Resource
    Electronic Resource
    The Naturally Transformable Marine Bacterium WJT-1C Formally Identified as “Vibrio” Is a Pseudomonad (1996)
    Springer
    Current microbiology 33 (1996), S. 287-291 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. A marine bacterial isolate, previously identified as Vibrio WJT-1C (ATCC 55351) and used as a model for investigating the process of natural transformation in the marine environment, has been further examined to determine its taxonomic identity. API 20E test strips, phenotypic testing, and flagellar staining had previously assigned the strain to the genus Vibrio, most closely related to V. campbelli. 16S rRNA analysis indicated that WJT-1C was in the Pseudomonas subgroup of the gamma proteobacteria. Bacteriophage typing and natural transformation with chromosomal DNA indicated that it was distinct from previously described marine transforming pseudomonads including Pseudomonas stutzeri strain JM300. The importance and abundance of the Pseudomonas subgroup of the gamma proteobacteria in the environment suggest that these marine strains are well suited as model organisms for describing the process and importance of natural transformation in nature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002849900115
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  • 10
    Electronic Resource
    Electronic Resource
    Phylogeny of Marine Bacillus Isolates from the Gulf of Mexico (2000)
    Springer
    Current microbiology 41 (2000), S. 84-88 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The phylogeny of 11 pigmented, aerobic, spore-forming isolates from marine sources was studied. Forty-two biochemical characteristics were examined, and a 16S rDNA sequence was obtained for each isolate. In a phylogenetic tree based on 16S sequencing, four isolates (NRRL B-14850, NRRL B-14904, NRRL B-14907, and NRRL B-14908) clustered with B. subtilis and related organisms; NRRL B-14907 was closely related to B. amyloliquefaciens. NRRL B-14907 and NRRL B-14908 were phenotypically similar to B. amyloliquefaciens and B. pumilus, respectively. Three strains (NRRL B-14906, NRRL B-14910, and NRRL B-14911) clustered in a clade that included B. firmus, B. lentus, and B. megaterium. NRRL B-14910 was closely related phenotypically and phylogenetically to B. megaterium. NRRL B-14905 clustered with the mesophilic round spore-producing species, B. fusiformis and B. sphaericus; the isolate was more closely related to B. fusiformis. NRRL B-14905 displayed characteristics typical of the B. sphaericus-like organisms. NRRL B-14909 and NRRL B-14912 clustered with the Paenibacillus species and displayed characteristics typical of the genus. Only NRRL B-14851, an unusually thin rod that forms very small spores, may represent a new Bacillus species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002840010098
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