ISSN:
1433-4909
Keywords:
Key words Peptidase
;
Biotransformations
;
Thermostability
;
Thermococcus
;
Catalytic triad
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The gene encoding pyrrolidone carboxyl peptidase (Pcp) has been cloned from the hyperthermophilic archaeon Thermococcus litoralis. The recombinant enzyme has been expressed in Escherichia coli, purified, and char-acterized. The T. litoralis Pcp demonstrates strong sequence homology to previously characterized bacterial Pcps. Some investigations have been carried out on enzyme substrate specificity and stability.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s007920070017
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