ISSN:
1435-1536
Keywords:
Key words Electron Paramagnetic Resonance
;
EPR
;
microemulsions
;
reverse micelles
;
AOT
;
lipase
;
esterification
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Abstract Structural and catalytic properties of cutinase were studied in bis(2-ethylhexyl) sodium sulfo-succinate (AOT)-isooctane microemulsion systems. The effect of the water content of the microemulsions on the cutinase activity on an esterification reaction of lauric acid with pentanol showed that cutinase followed a bell-shaped profile presenting a maximum at w o=9, with w o=[H2O]/[AOT]. Kinetic studies allowed the determi-nation of the apparent parameters K m and V max. Electron paramagnetic resonance (EPR) spectroscopy studies of active site labeled cutinase in microemulsions with varying w o values showed that in all microemulsions, the mobility of the label is higher than in the aqueous solution. Furthermore, it was found that the maximum of the enzyme activity did not correspond to a reduced active site mobility. Up to w o=9 there was an increase of both activity and active site mobility. As the water content of the system became higher, the mobility of the bound spin label further increased whereas the enzymatic activity dropped considerably.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s003960050126
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