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  • 1
    Electronic Resource
    Electronic Resource
    Silicon and the ecology of marine plankton diatoms. II. Silicate-uptake kinetics in five diatom species (1973)
    Springer
    Marine biology 19 (1973), S. 262-269 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The variation of the rate of silicate uptake with varying silicate concentration in the medium was investigated in short-term experiments with the following marine diatom species:Skeletonema costatum, Thalassiosira pseudonana, T. decipiens, Ditylum brightwellii, andLicmophora sp. The uptake conformed to Michaelis-Menten kinetics only after a correction had been made for reactive silicate that apparently could not be utilized by the diatoms. The magnitude of this correction was in the range of 0.3 to 1.3 μg-at Si/l. Mean values of the half-saturation constant of silicate uptake were calculated for the different species. The lowest value was found inS. costatum (0.80 μg-at Si/l) and the highest inT. decipiens (3.37 μg-at Si/l). Growth limitation by low silicate concentrations could be a cause of species succession in marine plankton-diatom blooms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02097147
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  • 2
    Electronic Resource
    Electronic Resource
    Ammonium regeneration by microzooplankton in the Oslofjord (1982)
    Springer
    Marine biology 69 (1982), S. 55-63 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Concentrations of dissolved inorganic nitrogen compounds above the pycnocline in the Oslofjord are very low in the summer, with turnover times of the inorganic N pools of no more than a few hours. To investigate the possibility that continued phytoplankton growth in the summer depends on ammonium excretion by microzooplankton, rates of NH 4 + regeneration and assimilation were measured by a 15N isotope dilution method. Daytime regeneration rates at 0–2 m depth were 0–28% of the calculated assimilation rates at ambient NH 4 + concentrations. Regeneration was faster during a dinoflagellate bloom in August than in mixed diatom-dinoflagellate blooms in June and September. Most of the NH 4 + appeared to be produced by juvenile copepods, rotifers, tintinnids, and heterotrophic dinoflagellates in the size fraction 45–200 μm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00396961
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  • 3
    Electronic Resource
    Electronic Resource
    Silicon and the ecology of marine plankton diatoms. I. Thalassiosira pseudonana (Cyclotella nana) grown in a chemostat with silicate as limiting nutrient (1973)
    Springer
    Marine biology 19 (1973), S. 117-126 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The small marine plankton diatom Thalassiosira pseudonana Hasle and Heimdal (Guillard's clone 3H) was grown in chemostats with silicate as the limiting nutrient. The calculated maximum growth rates were comparable to those previously reported for this species. The silica content of the diatom shells varied with the growth rate. As the growth rate approached zero, there were still measurable quantities of residual reactive silicate in the medium. In one of the two chemostats used, silicate assimilation by the cells was inefficient due to some unknown internal or external factor. In the other chemostat, statistically calculated half-saturation constants of growth were in the range of 0.5 to 0.8 μg—at Si/l, depending on which kind of correction was made for residual silicate. Half-saturation constants of steady-state mean silicate uptake per cell and hour, calculated in a similar fashion, were in the range of 1.4 to 2.6 μg—at Si/l. These results indicate that the silicate concentrations causing a reduced silicate uptake by this species in nature do not necessarily result in a correspondingly reduced growth rate. Growth in coastal waters is likely to become seriously limited by a shortage of silicate only when most of the silicate originally present has been removed in the course of a diatom bloom.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00353582
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  • 4
    Electronic Resource
    Electronic Resource
    Light-dependent coccolith formation in the two forms of Coccolithus pelagicus (1969)
    Springer
    Archives of microbiology 67 (1969), S. 199-208 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two methods were employed for measuring coccolith formation and photosynthesis in coccolithophorids. The first method was based on measurements of 14C radioactivity of cells on membrane filters before and after acid treatment. The second method involved a conversion of 14C in coccoliths or whole cells to BaCO3 prior to counting. It was observed that in determinations of photosynthetic (or total) 14C by the first method, the count rate produced by a given amount of the isotope was 30–40% lower in the non-motile and motile forms of Coccolithus pelagicus than in C. huxleyi. There was no similarly great discrepancy in determinations of coccolith 14C. Light-dependent coccolith formation was demonstrated in both forms of C. pelagicus. The non-motile form may deposit several times more carbon in its coccoliths than it assimilates photosynthetically. In the motile form, coccolith carbon amounts to less than 2% of photosynthetic carbon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409686
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  • 5
    Electronic Resource
    Electronic Resource
    A physiological comparison of coccolith-forming and naked cells of Coccolithus huxleyi (1970)
    Springer
    Archives of microbiology 73 (1970), S. 143-152 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In a naked clone of the coccolithophorid Coccolithus huxleyi, growth at light saturation was 15% slower than in a coccolith-forming clone isolated from the same parent stock. The two cell types did not differ significantly with regard to cell volume and protein content. A 10–13% smaller chlorophyll a content of the naked cells was matched by a uniform lowering of photosynthetic rates at all light intensities. It was considered that the slower growth of these cells might result from a less favourable ratio between photosynthetic output and biomass. The content of deoxyribonucleic acid was the same in coccolith-forming and naked cells, suggesting that they do not represent different phases in a sexual lifecycle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410317
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  • 6
    Electronic Resource
    Electronic Resource
    Two different Coccolithus huxleyi cell types incapable of coccolith formation (1971)
    Springer
    Archives of microbiology 75 (1971), S. 382-385 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407700
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  • 7
    Electronic Resource
    Electronic Resource
    Coccolith Formation (1962)
    [s.l.] : Nature Publishing Group
    Nature 193 (1962), S. 1094-1095 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] In young cultures of Coccolithus huocleyi (Lohm.) Kamptn., I have on numerous occasions observed one (Fig. 1) or two coccoliths in the cytoplasm between the two chromatophores. Their actual liberation has not been observed, but the following experiment suggests that the average time needed by the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/1931094b0
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  • 8
    Electronic Resource
    Electronic Resource
    Nitrogen nutrition of the phytoplankton in the Oslofjord
    Amsterdam : Elsevier
    Estuarine and Coastal Marine Science 14 (1982), S. 237-249 
    Details
    ISSN: 0302-3524
    Keywords: Oslo Fjord ; ammonium ions ; emission spectroscopy ; nitrates ; plankton ; seasonal variations ; temperature
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Geography , Geosciences
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0302-3524(82)80014-5
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  • 9
    Electronic Resource
    Electronic Resource
    Biology and Physiology of Coccolithophorids (1968)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Microbiology 22 (1968), S. 71-86 
    Details
    ISSN: 0066-4227
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.mi.22.100168.000443
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  • 10
    Electronic Resource
    Electronic Resource
    Effect of Ammonia and Nitrate on Growth, Photosynthesis, and Ribulosediphosphate Carboxylase Content of Dunaliella tertiolecta (1971)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 25 (1971), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: When the marine Chlorophycean flagellate Dunaliella tertiolecta Butcher was grown with short photoperiods of bright light, the use of ammonia rather than nitrate as a nitrogen source led to a 30 % reduction of the doubling time of cell matter. The cell cycle (onset of light to completion of cell division) was shortened by about 10% only. Ammonia-grown cells possessed a greater capacity for photosynthetic oxygen evolution at light saturation than did nitrate-grown cells; their content of ribulosediphosphate carboxylase was likewise greater. The faster growth of Dunaliella tertiolecta with ammonia may be partly a consequence of a general increase in net protein synthesis resulting in a greater content of photosynthetic enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1971.tb01445.x
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