Publication Date:
2001-02-07
Description:
The requirement for processing glycolipid antigens in T cell recognition was examined with mouse CD1d-mediated responses to glycosphingolipids (GSLs). Although some disaccharide GSL antigens can be recognized without processing, the responses to three other antigens, including the disaccharide GSL Gal(alpha1--〉2)GalCer (Gal, galactose; GalCer, galactosylceramide), required removal of the terminal sugars to permit interaction with the T cell receptor. A lysosomal enzyme, alpha-galactosidase A, was responsible for the processing of Gal(alpha1--〉2)GalCer to generate the antigenic monosaccharide epitope. These data demonstrate a carbohydrate antigen processing system analogous to that used for peptides and an ability of T cells to recognize processed fragments of complex glycolipids.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Prigozy, T I -- Naidenko, O -- Qasba, P -- Elewaut, D -- Brossay, L -- Khurana, A -- Natori, T -- Koezuka, Y -- Kulkarni, A -- Kronenberg, M -- AI40617/AI/NIAID NIH HHS/ -- CA52511/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 2001 Jan 26;291(5504):664-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11158680" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Motifs
;
Animals
;
Anti-Bacterial Agents/pharmacology
;
*Antigen Presentation
;
Antigen-Presenting Cells/immunology
;
Antigens, CD1/chemistry/*immunology/metabolism
;
Antigens, CD1d
;
Carbohydrate Conformation
;
Cytokines/biosynthesis
;
Epitopes/immunology
;
Galactosylceramides/chemistry/*immunology/*metabolism
;
Lysosomes/enzymology
;
*Macrolides
;
Mice
;
Mice, Inbred C57BL
;
Protein Transport
;
Receptors, Antigen, T-Cell/immunology
;
T-Lymphocytes/*immunology
;
Transfection
;
Tumor Cells, Cultured
;
alpha-Galactosidase/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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