Publication Date:
2011-05-28
Description:
Selective autophagy can be mediated via receptor molecules that link specific cargoes to the autophagosomal membranes decorated by ubiquitin-like microtubule-associated protein light chain 3 (LC3) modifiers. Although several autophagy receptors have been identified, little is known about mechanisms controlling their functions in vivo. In this work, we found that phosphorylation of an autophagy receptor, optineurin, promoted selective autophagy of ubiquitin-coated cytosolic Salmonella enterica. The protein kinase TANK binding kinase 1 (TBK1) phosphorylated optineurin on serine-177, enhancing LC3 binding affinity and autophagic clearance of cytosolic Salmonella. Conversely, ubiquitin- or LC3-binding optineurin mutants and silencing of optineurin or TBK1 impaired Salmonella autophagy, resulting in increased intracellular bacterial proliferation. We propose that phosphorylation of autophagy receptors might be a general mechanism for regulation of cargo-selective autophagy.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3714538/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3714538/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wild, Philipp -- Farhan, Hesso -- McEwan, David G -- Wagner, Sebastian -- Rogov, Vladimir V -- Brady, Nathan R -- Richter, Benjamin -- Korac, Jelena -- Waidmann, Oliver -- Choudhary, Chunaram -- Dotsch, Volker -- Bumann, Dirk -- Dikic, Ivan -- 250241/European Research Council/International -- New York, N.Y. -- Science. 2011 Jul 8;333(6039):228-33. doi: 10.1126/science.1205405. Epub 2011 May 26.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Frankfurt Institute for Molecular Life Sciences and Institute of Biochemistry II, Goethe University School of Medicine, Frankfurt (Main), Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21617041" target="_blank"〉PubMed〈/a〉
Keywords:
Adaptor Proteins, Signal Transducing/metabolism
;
*Autophagy
;
Cell Line, Tumor
;
Cytosol/*microbiology
;
HeLa Cells
;
Humans
;
Immunity, Innate
;
Microtubule-Associated Proteins/metabolism
;
Models, Biological
;
Nuclear Proteins/chemistry/metabolism
;
Phosphorylation
;
Protein Binding
;
Protein Interaction Domains and Motifs
;
Protein-Serine-Threonine Kinases/metabolism
;
RNA Interference
;
Salmonella typhimurium/*growth & development/immunology
;
Transcription Factor TFIIIA/chemistry/genetics/*metabolism
;
Ubiquitin/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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