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  • 1
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The responses of 24 white yam (Dioscorea rotundata) cultivars to mechanical and vector transmission with each of three viruses infecting yams were assessed through enzyme-linked immunosorbent assay (ELISA) and symptom development. The viruses were Dioscorea alata virus (DAV), genus Potyvirus; Dioscorea alata bacilliform virus (DaBV), genus Badnavirus; and Cucumber mosaic virus (CMV), genus Cucumovirus. Only TDr 95-128, a landrace cultivar from Nigeria, developed symptoms of infection with CMV and DaBV following mechanical and vector transmission, respectively. PAS-ELISA showed that nine genotypes remained uninfected by DAV and 11 were uninfected by CMV or DaBV. Genotypes TDr 747 and TDr 1640 both showed resistance to all three viruses.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Sexual plant reproduction 5 (1992), S. 195-200 
    ISSN: 1432-2145
    Keywords: Interspecific hybridization ; Vigna unguiculata ; V. vexillata ; Pollen-pistil incompatibility ; Embryo abortion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Interspecific hybridization between Vigna unguiculata and V. vexillata always failed: no seed was obtained in both crossing directions. Two different barriers to crossability were found: a pre-zygotic barrier and a post-zygotic one. Many abnormalities were observed in pollen-tube development, which reduced the percentage of fertilization to 18–30%. Differences in the percentage of fertilization were detected between the two accessions of V. vexillata involved in the interspecific crosses. The development of the interspecific embryo was analyzed and the embryo and endosperm nuclei always degenerated 5–8 days after pollination. The growth of the embryo stopped at a globular stage, which is too early for excision and in vitro culturing.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Genetic resources and crop evolution 43 (1996), S. 493-504 
    ISSN: 1573-5109
    Keywords: flavonoids ; HPLC fingerprints ; infra- and interspecific relationships ; Vigna taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Flavonoid chemical markers together with other biochemical markers and/or morphological data can provide useful characters for defining species in Vigna genus. In this paper flavonid HPLC fingerprints have been utilized to assess taxonomic relationships amongst species and accessions of Vigna. Within the taxa analysed there is evidence of both infra- and interspecific chemical variations. In some species chromatographic data support evidence for the existence of different flavonoid chemotypes, which may reflect differences in geographic origin. As far as interspecific relationships are concerned, species belonging to sections Vigna, Plectotropis and Ceratotropis do not show flavonoid glycosides in common with cultivated lines of Vigna. By contrast, some relationships have been found between cultivated lines and wild species of section Catiang. Kaempferol-3-rutinoside and an unidentified kaempferol-glycoside were the only flavonoids detected in the three subgenera analysed. Finally, a greater variability in flavonoid aglycone class and glycosylation pattern has been observed in cultivars of V. unguiculata (L.) Walp. compared to the wild species. This observation may confirm that cultivation and/or domestication may cause or increase species diversification.
    Type of Medium: Electronic Resource
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  • 4
    Publication Date: 1996-12-01
    Print ISSN: 0925-9864
    Electronic ISSN: 1573-5109
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Springer
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  • 5
    Publication Date: 2002-02-01
    Description: Hermetic cold storage without previous drying (wet–cold storage) was experimented for the preservation of yam pollen grains. Pollens collected from white yam D. rotundata and a wild relative, D. praehensilis, were stored at −80, −20, 5, and 15 °C in sealed packs for 2 years. Pollen samples were drawn for in vitro germination tests after 100 and 700 days, and hand pollination was conducted after 365 and 730 days in storage. Pollen germination responses were not significantly different among the two species. Though pollen maintained germination capacity at all the storage temperature regimes, there was a significant loss in the viability of pollen stored at 5 and 15 °C after 100 days of storage. After 700 days in storage, pollens stored at 5 and 15 °C had lost germination capacity while there were no significant differences in the germination of fresh pollen and pollen frozen at −80 and −20 °C. Hand pollination with pollen of D. rotundata frozen at −80 °C for 365 days gave 69·5% fruit set and 50% fruit set after 730 days in storage. From these results, the wet–freeze procedure appears promising to execute the establishment of pollen gene banks for yam breeding and for conservation of haploid gene pool of yams in base collections. A pollen storage protocol based on the procedure is recommended.
    Print ISSN: 0021-8596
    Electronic ISSN: 1469-5146
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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