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  • 1
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26829 | 23782 | 2019-11-19 01:08:33 | 26829 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: The paper provides the methodology in lipid extraction from fish using the Modified Folch's Method. Instructions on sample preparation, and the apparatus and reagents needed are presented. Detailed procedures in lipid extraction are provided.
    Keywords: Chemistry ; Fisheries ; Manuals ; Culture media ; Analysis ; Analytical techniques ; Methodology ; Chemical analysis ; Fish inspection ; Fishery industry ; Food technology ; Standards ; Specifications ; Lipids
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: C-2.1-C-2.2
    Format: 2
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  • 2
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26832 | 23782 | 2019-11-19 00:56:39 | 26832 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: The paper provides the methodology in determination of free fatty acids (FFA) in fish.
    Keywords: Chemistry ; Fisheries ; Manuals ; Culture media ; Analysis ; Analytical techniques ; Methodology ; Chemical analysis ; Food technology ; Standards ; Specifications ; Fatty acids
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: C-5.3
    Format: 1
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  • 3
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26838 | 23782 | 2019-11-19 00:36:53 | 26838 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: The paper provides the methodology in the preparation of methyl esters by boron trifluoride method in fish. Instructions on sample preparation and the apparatus and reagents needed are presented. Detailed procedures are also provided.
    Keywords: Chemistry ; Fisheries ; Manuals ; Culture media ; Analysis ; Analytical techniques ; Methodology ; Chemical analysis ; Food technology ; Standards ; Specifications ; Fish oils ; Fats ; Fatty acids ; Esters ; Lipids
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: C-11.1-C-11.2
    Format: 2
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  • 4
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26835 | 23782 | 2019-11-19 00:46:29 | 26835 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: The paper provides the methodology in the determination of thiobarbituric acid (TBA) number in fish fat. The apparatus and reagents needed are presented. Detailed procedures and calculations are also presented.
    Keywords: Chemistry ; Fisheries ; Manuals ; Culture media ; Analysis ; Analytical techniques ; Methodology ; Chemical analysis ; Food technology ; Standards ; Specifications ; Fish oils ; Fats ; Fatty acids
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: C-8.1-C-8.3
    Format: 3
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  • 5
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26837 | 23782 | 2019-11-19 00:39:35 | 26837 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: The paper provides the methodology in the determination of the degree of lipid oxidation by gas chromatography. Instructions on sample preparation and the detailed procedures and calculation are provided.
    Keywords: Chemistry ; Fisheries ; Manuals ; Culture media ; Analysis ; Analytical techniques ; Methodology ; Chemical analysis ; Food technology ; Standards ; Specifications ; Lipids ; Fats ; Fatty acids ; Oxidation ; Chromatographic techniques
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: C-10.1
    Format: 1
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  • 6
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26743 | 23782 | 2019-09-12 01:49:08 | 26743 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: The K value, expressed as K value % = HxR + Hx /ATP + ADP + AMP + IMP + HxR + Hx x 100 is a good index for estimating the enzymatic freshness of fish. The changes in K value of various species of tropical food fishes during ice-storage had been studied. It was found that these warm water fishes deteriorate slowly under ice-storage preservation. A study was made on the K value changes of three species of fish (Polynemus sp., Rastrelliger kanagurta and Pampus argenteus) in a supermarket distribution chain. Most sets of data showed changes of quality; from the point of supply until the morning after an overnight stay on display shelves. The results support a conclusion that the shelf life limit of fresh tropical food fish, based on sensory evaluation, was, when handled well, between 12 to 28 days ice-storage depending on species, with the K value ranging from 24 -37%.
    Keywords: Fisheries ; Food fish ; Tropical fish ; Quality control ; Freezing storage
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: 250-257
    Format: 8
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  • 7
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26803 | 23782 | 2019-11-12 05:32:47 | 26803 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: Presented in the paper is the standard procedure in the measurement of free and expressible drips. Specifically, the required apparatus, and the analytical procedures and calculations for free and expressible drips are presented.
    Keywords: Chemistry ; Fisheries ; Manuals ; Culture media ; Analysis ; Analytical techniques ; Methodology ; Chemical analysis ; Microbiological analysis ; Fishery products ; Processed fishery products ; Fish inspection ; Fishery industry ; Food technology ; Standards ; Specifications
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: A-4.1-A-4.2
    Format: 2
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  • 8
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26742 | 23782 | 2019-09-13 00:34:51 | 26742 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: Initial investigations into the use of Sardinella gibbosa for making surimi showed that gel strength (G.S.) of around 400 g cm was achievable under normal surimi processing conditions. Adjusting the pH during the leaching process, by means of NaHCO3 and by means of Na4O7P2 with vacuum, did not improve the G.S. The fat content of local sardine was low and did not interfere with the surimi processing. The optimum conditions for setting the gel of paste were subjected to between 40 to 50° C for 20 min, followed by 20 min at 90°C. The surimi underwent modori* when subjected to 60°C for 20 min. Sugar was necessary as a cryoprotective agent for frozen surimi. It was found that crude aqueous extract of unfrozen S. gibbosa kidney tissues had G.S. enhancing effect. Kidney extract made from frozen sardine which were then frozen again, lost this G.S. enhancing effect. Kidney extract made from unfrozen Caesio erythrogaster also had this G.S. enhancing effect. The kidney extract was heat stable, and retained the G.S. enhancing effect after exposure to 80°C for 10 min. However, the kidney extract did not prevent modori when the gel was exposed to 60°C for 20 min.
    Keywords: Fisheries ; Sardinella gibbosa ; Minced products ; Processing fishery products ; Freezing ; Storage effects
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: 236-249
    Format: 14
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  • 9
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26744 | 23782 | 2019-09-13 00:44:22 | 26744 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: Boiled, dried anchovies sold in Singapore are mainly imported from neighbouring countries. A survey was conducted to obtain an understanding of the quality of this product which is sold packed in polypropylene bags at supermarkets. The survey showed that the product had a high moisture content (=25% dry wt) and hence should be categorised as an intermediate-moisture product rather than as a dried product. It also had a high percentage breakage, a considerable amount of foreign matter, high water activity from 0.663 to 0.700 and total lipid of 7.0 - 8.6%. The organoleptic quality of most samples was acceptable. This survey showed that the quality of this product at retail outlets could be improved. The following are suitable quality indicators: percentage breakage, protein content, salt content, ash and acid insoluble ash, but they need not be monitored in storage studies. The suitable quality indices for quality changes of this product were identified as moisture content, water activity, total volatile basic nitrogen, total lipid, peroxide value, thiobarbituric acid number, colour and sensory evaluation. Trimethylamine nitrogen is, however not a suitable parameter. The effect of storage at different conditions (refrigerated: 7±2 C, 80% RH; room temperature exposed to light and kept in the dark: 27±40°C, 73±16% RH) on the shelflife of the product was also investigated. The samples were again found to have a high moisture and hence mould growth was the main contributor to spoilage at room temperature. Refrigerated storage extended the shelflife beyond six weeks, yielding a fairly good quality product. However it caused the moisture and water activity to increase with storage. The shelflife of samples stored at room temperature exposed to light was 4 weeks and those in the dark was two weeks. Drying and vacuum packing were done to study their effect on shelflife extension. The samples were further dried to a moisture of =25% (dry weight) using the oven and low temperature vacuum dryer, then vacuum packed and stored in an air-conditioned room. These were compared with two other samples that were not further dried; one lot was stored exposed at room temperature and the other was vacuum packed and stored in an air-conditioned room. The samples were monitored weekly for the first 6 weeks and thereafter monthly. The first 6 weeks of this experiment is reported here; these preliminary results showed that further drying and vacuum packaging effectively reduced the moisture and also retarded mould growth. Thus the product is still fairly good even after six weeks.
    Keywords: Fisheries ; Dried products ; Water content ; Quality control ; Storage conditions ; Storage life ; Evaluation ; Packing fishery products
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: 258-272
    Format: 15
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  • 10
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26822 | 23782 | 2019-11-21 01:02:52 | 26822 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: Presented in the paper is the standard procedure in the determination of DMA-N by Dyer's colometric method using copper dithiocarbamate in fish meat. Specifically, the reagents needed and the analytical procedures in the determination of DMA are presented.
    Keywords: Chemistry ; Fisheries ; Manuals ; Culture media ; Analysis ; Analytical techniques ; Methodology ; Chemical analysis ; Fishery products ; Fish inspection ; Fishery industry ; Food technology ; Standards ; Specifications
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: B-4.1-B-4.4
    Format: 4
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