ISSN:
1432-0886
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Giemsa C-banding of two liverwort species, Pellia epiphylla (L.) Corda (n=9) and P. neesiana (Gott.) Limpr. (n = X/Y + 8), identified each chromosome as unique. Almost the full complement of C-bands was defined by N-banding. Apart from minor variation in the prominence of certain bands, which were designated according to principles adopted in human cytology, the chief exceptions were the absence of bands 1p2, 1p4, 1q2, 1q4 and 7p6 from the N-banded karyotype of P. epiphylla and of band 7q25 from P. neesiana. Both quinacrine dihydrochloride and Hoechst 33258 showed reduced fluorescence in the constitutive heterochromatin of P. epiphylla. The effects of these two fluorochromes on P. neesiana, however, differed from each other and from their effect on P. epiphylla. Only one Q-band, the brightly fluorescing 1(X/Y)q24, occurred, whereas Hoechst 33258 resulted in considerable differentiation. The majority of C-bands were correlated with bright fluorescence with Hoechst 33258 but a few were dim. On the basis of these results, four major types of heterochromatin were identified in P. neesiana and two further types in P. epiphylla. They are discussed in the context of previously reported early replication of P. neesiana heterochromatin and point to considerable cytological evolution within these two species.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00327470
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