ISSN:
1573-5168
Keywords:
fish EGF
;
receptor
;
tyrosine kinase
;
western blot
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The association between the presence of EGF binding and its associated tyrosine kinase activity was investigated in the hepatic membranes of rainbow trout (Oncorhynchus mykiss). Time course experiments indicted that the binding of EGF to hepatic membranes was temperature dependent and resulted in increased phosphorylation of membrane components. The phosphorylation reaction was dependent on the presence of divalent ions (Mg2+ and Mn2+) but not monovalent ions (Na+ and K+) or calcium. Western blot analysis of phosphorylated trout membranes indicated protein bands at 150 and 180 kDa which is similar to that observed in mammals. Use of specific substrates and protein kinase inhibitors also indicated that the increase in EGF stimulated phosphorylation was related to an increase in tyrosine kinase activity. These data support the hypothesis that trout have an EGF-like receptor that has binding and biological activities similar to that observed in mammalian species.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1007872209650
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