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  • 1
    Electronic Resource
    Electronic Resource
    Bradford : Emerald
    British food journal 103 (2001), S. 443-452 
    ISSN: 0007-070X
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sets out to consider the way in which food retailing has altered in the face of competitive and other forces. Specifically, the space allocated in respect of the mix of food and non-food product areas, and the redistribution of priorities as traditional retailer policies and strategies become transformed by consumer demands. Discusses large food supermarket chains to provide a context for development. A framework of four major factors, time, location, space and merchandising, is used to help to identify and analyse key factors in the customers' and retailers' expectations and strategic thought behind the merchandise arrangements.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Bradford : Emerald
    British food journal 102 (2000), S. 398-405 
    ISSN: 0007-070X
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: This research focuses on the UK fast food retailers and the importance of the service delivery systems in fast food burger outlets. Waiting time and the impact it has on customer perceptions of service quality is considered alongside a typology of customers, based on their waiting characteristics. A number of critical components that affect customer queuing and crowding emerge as an inherent part of the production-line service system. The paper asserts that the cost-effective maximisation of service speed is likely to be the primary consideration for management, and lead to business success. When using conventional research approaches, such as tracking studies and queuing theory, these arguably offer burger restaurant managers a rather simplistic analysis. The research concludes, therefore, that modern computer-based simulation packages offer a way of measuring most of the influencing factors, and is an opportunity for leading fast food retailers to optimise their (total) product positioning.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Bingley : Emerald
    International journal of retail and distribution management 31 (2003), S. 591-600 
    ISSN: 0959-0552
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Economics
    Notes: Store layouts are important determinants of behaviour. A review of the academic and commercial literature suggests that the methodological and theoretical approaches have provided methods and approaches that are difficult for practitioners to adopt. This paper offers a robust theoretical approach (the behavioural perspective model) and new innovative methodology that significantly advances the way retailers can plan and measure store layouts, with a view to optimising store performance. Using computer-aided observation, customers may be tracked and their behaviour analysed in the context of consumer situations and contingencies. Implications for retail management, theory and practice are discussed within the context of fashion shopper situations.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Bradford : Emerald
    European journal of marketing 38 (2004), S. 770-789 
    ISSN: 0309-0566
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Economics
    Notes: Topshop and Gap are important fashion retail brands targeting young style-conscious UK consumers. However, business performance differs widely with Topshop enjoying record sales whilst Gap has recorded losses at a five year high. This paper investigated these variations in performance from a strategic and holistic view of retailer brand image, and the adoption of quality marketing orientation. Answers were sought using key image attributes as a vehicle to understanding customers' perceptions of the retailers under study. Survey data were collected from a sample of 300 typical customers who were set the task of ranking image attributes. Factor analysis of customer responses indicated that Gap customers were offered lifestyles in advertising that were out-of-step with the merchandise on the shelves. This reflected a failure on the part of retail management to satisfy the target market. Topshop customers displayed high levels of confidence in the merchandise proposition but stressed the importance of atmosphere and sales staff in overall assessments. This finding reinforces the view that sustainable retailer positioning pivots on a range of marketing activities as well as matching fashion consumers to styles. The research concludes with implications for strategic retail marketing, theory and practice.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The adjacent genes rpoB and rpoC code for the β and β′ subunits of RNA polymerase in Escherichia coli, and are cotranscribed in the order given. The nearest known genes to rpoB are rplL and rplA,J,K, which code for ribosomal proteins, and which are transcribed in the same direction as the polymerase genes. It has been suggested that rpoBC may be distal elements of a larger operon including these ribosomal genes. To test this possibility we have cloned a segment of DNA, derived by endoR. HindIII digestion from the rpoBC-transducing bacteriophage λrif d 18, in the replacement vector NMλ761. The structure of the λrpoBC bacteriophages so produced is such that the inserted DNA can be transcribed from λ promoters, allowing us to confirm that it carries intact rplL, rpoB, and rpoC genes. We have studied these bacteriophages as lysogens in rec + and rec bacteria, and by infection of UV-irradiated bacterial strains in which λ promoters are either repressed or active. The results indicate that the cloned DNA contains at most a very weak promoter for the above genes, in contrast to that present in the larger segment of bacterial DNA carried by λrif d 18. We have in the same way cloned the adjacent bacterial HindIII-fragment of λrif d 18 DNA, and have found that it displays vigorous autonomous expression of the tufB, rplA, and rplK genes. We conclude that rpoB and C are obligatorily co-transcribed with rplL, from a promoter located outside the DNA segment cloned in λrpoBC. We discuss the evidence for the existence of a regulatory site, rpoU, located between rplL and rpoB.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 184 (1981), S. 548-550 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Four ribosomal protein genes, rplA, rplJ, rplK and rplL form an operon in E. coli together with the genes rpoB and rpoC which encode the β and β′ subunits of RNA polymerase. Transcription is initiated principally at two promoters, P L11 and P1, the overall structure of the operon being (in the direction of transcription) P L11 rplK rplA P1 rplJ rplL rpoB rpoC. Here we describe studies of phage λ derivatives carrying various segments of this operon, which demonstrate the existence of at least three additional weak internal promoters, and help to define their positions and strengths.
    Type of Medium: Electronic Resource
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  • 7
    Publication Date: 2015-07-07
    Description: α-Synuclein (αS) is a highly abundant neuronal protein that aggregates into β-sheet–rich inclusions in Parkinson’s disease (PD). αS was long thought to occur as a natively unfolded monomer, but recent work suggests it also occurs normally in α-helix–rich tetramers and related multimers. To elucidate the fundamental relationship between αS multimers and monomers in living neurons, we performed systematic mutagenesis to abolish self-interactions and learn which structural determinants underlie native multimerization. Unexpectedly, tetramers/multimers still formed in cells expressing each of 14 sequential 10-residue deletions across the 140-residue polypeptide. We postulated compensatory effects among the six highly conserved and one to three additional αS repeat motifs (consensus: KTKEGV), consistent with αS and its homologs β- and γ-synuclein all forming tetramers while sharing only the repeats. Upon inserting in-register missense mutations into six or more αS repeats, certain mutations abolished tetramer formation, shown by intact-cell cross-linking and independently by fluorescent-protein complementation. For example, altered repeat motifs KLKEGV, KTKKGV, KTKEIV, or KTKEGW did not support tetramerization, indicating the importance of charged or small residues. When we expressed numerous different in-register repeat mutants in human neural cells, all multimer-abolishing but no multimer-neutral mutants caused frank neurotoxicity akin to the proapoptotic protein Bax. The multimer-abolishing variants became enriched in buffer-insoluble cell fractions and formed round cytoplasmic inclusions in primary cortical neurons. We conclude that the αS repeat motifs mediate physiological tetramerization, and perturbing them causes PD-like neurotoxicity. Moreover, the mutants we describe are valuable tools for studying normal and pathological properties of αS and screening for tetramer-stabilizing therapeutics.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 8
    Publication Date: 2013-06-01
    Print ISSN: 0969-2126
    Electronic ISSN: 1878-4186
    Topics: Biology , Medicine
    Published by Cell Press
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  • 9
    Publication Date: 2018-03-01
    Description: Weather and climate variability strongly influence the people, infrastructure, and economy of Alaska. However, the sparse observational network in Alaska limits our understanding of meteorological variability, particularly of precipitation processes that influence the hydrologic cycle. Here, a new 14-yr (September 2002–August 2016) dataset for Alaska with 4-km grid spacing is described and evaluated. The dataset, generated with the Weather Research and Forecasting (WRF) Model, is useful for gaining insight into meteorological and hydrologic processes, and provides a baseline against which to measure future environmental change. The WRF fields are evaluated at annual, seasonal, and daily time scales against observation-based gridded and station records of 2-m air temperature, precipitation, and snowfall. Pattern correlations between annual mean WRF and observation-based gridded fields are r = 0.89 for 2-m temperature, r = 0.75 for precipitation, r = 0.82 for snow-day fraction, r = 0.55 for first snow day of the season, and r = 0.71 for last snow day of the season. A shortcoming of the WRF dataset is that spring snowmelt occurs too early over a majority of the state, due partly to positive 2-m temperature biases in winter and spring. Strengths include an improved representation of the interannual variability of 2-m temperature and precipitation and accurately simulated (relative to regional station observations) winter and summer precipitation maxima. This initial evaluation suggests that the 4-km WRF climate dataset robustly simulates meteorological processes and recent climatic variability in Alaska. The dataset may be particularly useful for applications that require high-temporal-frequency weather fields, such as driving hydrologic or glacier models. Future studies will provide further insight on its ability to represent other aspects of Alaska’s climate.
    Print ISSN: 1558-8424
    Electronic ISSN: 1558-8432
    Topics: Geography , Physics
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  • 10
    Publication Date: 2015-12-17
    Description: Continental-domain assessments of climate change impacts on water resources typically rely on statistically downscaled climate model outputs to force hydrologic models at a finer spatial resolution. This study examines the effects of four statistical downscaling methods [bias-corrected constructed analog (BCCA), bias-corrected spatial disaggregation applied at daily (BCSDd) and monthly scales (BCSDm), and asynchronous regression (AR)] on retrospective hydrologic simulations using three hydrologic models with their default parameters (the Community Land Model, version 4.0; the Variable Infiltration Capacity model, version 4.1.2; and the Precipitation–Runoff Modeling System, version 3.0.4) over the contiguous United States (CONUS). Biases of hydrologic simulations forced by statistically downscaled climate data relative to the simulation with observation-based gridded data are presented. Each statistical downscaling method produces different meteorological portrayals including precipitation amount, wet-day frequency, and the energy input (i.e., shortwave radiation), and their interplay affects estimations of precipitation partitioning between evapotranspiration and runoff, extreme runoff, and hydrologic states (i.e., snow and soil moisture). The analyses show that BCCA underestimates annual precipitation by as much as −250 mm, leading to unreasonable hydrologic portrayals over the CONUS for all models. Although the other three statistical downscaling methods produce a comparable precipitation bias ranging from −10 to 8 mm across the CONUS, BCSDd severely overestimates the wet-day fraction by up to 0.25, leading to different precipitation partitioning compared to the simulations with other downscaled data. Overall, the choice of downscaling method contributes to less spread in runoff estimates (by a factor of 1.5–3) than the choice of hydrologic model with use of the default parameters if BCCA is excluded.
    Print ISSN: 1525-755X
    Electronic ISSN: 1525-7541
    Topics: Geography , Geosciences , Physics
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