ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Two-dimensional electrophoresis and Western blotting with lectins were used to provide information about a glycoprotein without requiring its previous purification. The oestrus-associated glycoprotein from ovine oviductal fluid has an isoelectric point of 4.7 and a subunit molecular weight (Mr) range of 70 000-90 000. Under non-denaturing conditions, it is present as a high molecular weight aggregate or polymer although at low pH some monomer is also detected. The surface carbohydrate on this protein includes fucose, galactose and N-acetyl galactosamine residues but not glucose, mannose or sialic acid.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150061010
Permalink