ISSN:
0951-4198
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Physics
Notes:
Peptide analysis of cytochrome c using capillary electrophoresis/mass spectrometry was demonstrated to be a rapid, sensitive and useful technique for providing information concerning the location of variation. We analyzed tryptic digests of horse, bovine and dog cytochrome c, and compared the peptide analysis results for information concerning amino acid sequences in proteins. The differences in amino acid sequence were confirmed by taking into consideration that all the other fragments are identical in each map. The use of an untreated capillary column with basic buffer condition (water+acetonitrile+(20 g/L) ammonium carbonate (70/20/10), pH 9.2) had a reproducibility of migration times to within about 2%.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/rcm.1290080906
Permalink