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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 683 (1993), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 196 (1962), S. 774-775 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] An organism producing (3-r〉-l,6-glucanase was isolated from dust, using medium containing luteose as a sole carbon source. The taxonomic position of this isolated organism is as yet uncertain. The organism was grown in Czapek medium containing peptone and luteose as a sole carbon source at 30 C ...
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 86 (1992), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: To evaluate the role of pyrophosphate-dependent phosphofructokinase (PFP. EC 2.7.1.90) in the sugar metabolism of pollen. its occurrence and properties were studied in pollen grains of several plants including camellia (Camellia japonica L.). In all pollen samples, PFP was strongly activated by fructose-2,6-bisphosphate (F2,6BP), and the activity of F2,6BP-activated PFP was higher than that of phosphofructokinase (PFK. EC 2.7.1.11). PFP partially purified from camellia pollen required Mg2+ for activity with an optimum at 1 mM. and was almost unaflected by a variety of metabolites at 1 mM. Its molecular mass was around 220 kDa, and apparent Km values for F6P, PPi. F1, 6BP and Pi were 294, 4, 20 and 580 uM, respectively. The levels of F2.6BP. PPi and F6P in camellia pollen were sufficent to support the forward reaction by PFP, and PFP, was 20- to 40-fold more active than PFK during pollen growth. These results suggest that pollen PFP plays a role in glycolysis but not gluconeogenesis. and the possible relevance of this to pollen tube growth is discussed.
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 200 (1963), S. 1337-1338 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Previously2,3 we reported that a newly isolated microorganism produced lutease (b-l,6-glucan-6 glucano-hydrolase) when grown in a Czapek medium containing peptone and higher-molecular-weight luteose instead of sucrose. This latter substance is a b-D-l,6-glucan prepared from mucilage which was ...
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  • 5
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A three-month operation with a beam is scheduled for the autumn of 1995 at the TRISTAN MR for the sake of light-source development and research programs using it. The lattice will be modified so as to enable the installation of an undulator 5.4 m long and achieve a very low-emittance beam of 5 nm at 10 GeV. The emittance damping wigglers will enhance the radiation-damping rate, which will stabilize the coherent beam instabilities as well as reduce the emittance more. A considerable number of accelerating cavities will be removed from the ring in order to minimize the impedance of the higher-order modes of the cavities that may cause coupled-bunch instabilities and limit the intensity of the stored beam. For the stability of the light beam, a local feedback system will be applied to the electron orbit in the MR. The beam emittance will be measured by detecting the angular distribution of the Compton scattering of laser photons from the beam electrons. To measure the beam emittance in an independent way, visible synchrotron light from a bending magnet and 100 keV x rays from an undulator will be utilized. © 1995 American Institute of Physics.
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  • 6
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: Recently, a global feedback of beam position was switched on at SOR-RING of Tokyo University. At a low beam current, the vertical orbit drift was suppressed almost within a few μm and the horizontal orbit drift within several μm. On the other hand, the suppression became worse at a high current because of a longitudinal instability, which deteriorated the accuracy of the beam position monitor system. It was also observed that at 21 Hz, the repetition rate of the electron synchrotron close to SOR-RING, the horizontal orbit tends to vibrate uniformly around the ring, i.e., monopolelike expansion and contraction, while the vertical orbit has a dipolelike vibration; a result expected if a uniform magnetic field is impressed on the ring. © 1995 American Institute of Physics.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 81 (1991), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Extracts from Camellia japonica, Lilium longiflorum and L. lancifolium pollen grains showed a far higher kinase activity with fructose than with glucose. Fructokinase (EC 2.7.1.4) and hexokinase (EC 2.7.1.1) preparations were obtained by partial fractionation of the extracts by DEAE-cellulose chromatography; the former was practically inactive with glucose. All had a pH optimum at 7.0–8.0 and required Mg1+ ions for activity with optima at 0.5-1 mM and 1-2 mM for fructokinase and hexokinase activities, respectively. Fructokinases had Km of 0.2-0.4 mM for fructose and similar affinities for ATP and UTP, and were inhibited by fructose above 1 mM. Hexokinases had a higher affinity for glucose than for fructose and a lower affinity for UTP compared to ATP. In camellia pollen most of hexose kinase activities were found to be due to fructokinase. These results are discussed in relation to stimulation of camellia pollen tube growth by oligosaccharides susceptible to invertase (EC 3.2.1.26).
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  • 8
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] The Fas ligand (FasL), a member of the tumor necrosis factor family, induces apoptosis in Fas–bearing cells. The membrane–bound human FasL was found to be converted to a soluble form (sFasL) by the action of a matrix metalloproteinase–like enzyme. Two neutralizing monoclonal ...
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  • 9
    ISSN: 1432-203X
    Keywords: Carrot ; Pectin ; cell-to-cell attachment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cell walls and media were obtained from three kinds of carrot cell culture, namely, embryogenic callus (EC), non-embryogenic callus (NC) and somatic embryos (SE), and analyzed for their sugar content and sugar composition by electrophoresis and gas chromatography. EC formed large cell clusters while NC formed small clusters. Observations under the light microscope revealed that the intercellular contacts in NC were much more limited than those in EC. The analysis of pectic polysaccharides revealed that the level of neutral sugars was higher than that of acidic sugars in EC, while the opposite was true in NC. Gaschromatographic analysis of neutral sugars in pectic fractions revealed that EC and SE were rich in arabinose, while NC was rich in galactose. On the basis of these results, we discuss the possible involvement of neutral sugars, and of arabinose and galactose in particular, in pectic polysaccharides in intercellular contacts.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 206 (1999), S. 1-10 
    ISSN: 1615-6102
    Keywords: Arabinogalactan protein ; Cell wall component ; Immunocytochemistry ; Pectin ; Pinus densiflora ; Pollen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In order to compare cell wall formation in gymnosperm pollen with that in angiosperm pollen, the distribution of cell wall constituents in the pollen grain and pollen tube ofPinus densiflora was studied immunocytochemically with monoclonal antibodies JIM 5 (against non- or poorly esterified pectin), JIM 7 (against highly esterified pectin), JIM 13 (against arabinogalactan proteins, AGPs), and LM 2 (against AGPs containing glucuronic acid). In the pollen grain wall, only the outer layer of the intine was labeled with JIM 5 and weakly with JIM 7. The tube wall was scarcely labeled with JIM 5 and very weakly labeled with JIM 7. In contrast, the whole of both the intine and the tube wall was strongly labeled with JIM 13 and LM 2, and the generative-cell wall was also labeled only with LM 2. The hemicellulose B fraction, which is the main polysaccharide fraction from the pollen tube wall, reacted strongly with JIM 13 and especially LM 2, but not with antipectin antibodies. These results demonstrate that the wall constituents and their localization inP. densiflora pollen are considerably different from those reported in angiosperm pollen and suggest that the main components of the cell wall ofP. densiflora pollen are arabinogalactan and AGPs containing glucuronic acid.
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