Publication Date:
2009-08-22
Description:
We recently reported the chemical synthesis, assembly, and cloning of a bacterial genome in yeast. To produce a synthetic cell, the genome must be transferred from yeast to a receptive cytoplasm. Here we describe methods to accomplish this. We cloned a Mycoplasma mycoides genome as a yeast centromeric plasmid and then transplanted it into Mycoplasma capricolum to produce a viable M. mycoides cell. While in yeast, the genome was altered by using yeast genetic systems and then transplanted to produce a new strain of M. mycoides. These methods allow the construction of strains that could not be produced with genetic tools available for this bacterium.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lartigue, Carole -- Vashee, Sanjay -- Algire, Mikkel A -- Chuang, Ray-Yuan -- Benders, Gwynedd A -- Ma, Li -- Noskov, Vladimir N -- Denisova, Evgeniya A -- Gibson, Daniel G -- Assad-Garcia, Nacyra -- Alperovich, Nina -- Thomas, David W -- Merryman, Chuck -- Hutchison, Clyde A 3rd -- Smith, Hamilton O -- Venter, J Craig -- Glass, John I -- New York, N.Y. -- Science. 2009 Sep 25;325(5948):1693-6. doi: 10.1126/science.1173759. Epub 2009 Aug 20.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19696314" target="_blank"〉PubMed〈/a〉
Keywords:
Centromere
;
*Cloning, Molecular
;
DNA Methylation
;
DNA Restriction Enzymes/genetics/metabolism
;
Deoxyribonucleases, Type III Site-Specific/genetics
;
*Gene Transfer Techniques
;
*Genetic Engineering
;
*Genome, Bacterial
;
Mycoplasma capricolum/*genetics
;
Mycoplasma mycoides/*genetics/growth & development/isolation & purification
;
Plasmids
;
Saccharomyces cerevisiae/*genetics
;
Sequence Analysis, DNA
;
Sequence Deletion
;
Transformation, Bacterial
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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