ISSN:
1617-4623
Keywords:
Key words Stringent/relaxed response
;
Bacteriophage λpR promoter
;
Replication of λ plasmids
;
dnaA
;
Guanosine 5′-diphosphate-3′-diphosphate
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract It was previously demonstrated that the activity of bacteriophage λ promoter p R is decreased in wild-type Escherichia coli cells starved for amino acids (during the stringent response). Since p R activity is necessary for the transcriptional activation of oriλ, this leads to inhibition of the replication of plasmids derived from phage λ. These results led to the proposal that the p R promoter is susceptible to control by the stringent response. However, subsequent studies demonstrated that this promoter is activated by the host dnaA gene product and since the dnaA promoter was reported to be controlled by the stringent response, it is possible that the inhibition of p R activity in amino acid-starved cells is indirect, and results from the impairment of DnaA-mediated transcriptional activation. Here we present evidence that p R is negatively regulated by ppGpp, even when DnaA protein is provided in excess as well as in cells devoid of DnaA function. We have checked that the level of ppGpp is increased during prolonged (up to 4 h) starvation for isoleucine in relA + cells but not in the relA − mutant. At the same time we observed inhibition of λ plasmid replication during the stringent, but not relaxed, response, even when DnaA was overproduced. Finally, we found that the activity of a p R-lacZ fusion is inhibited after gratuitously induced overproduction of ppGpp in unstarved cells, irrespective of the status of the dnaA gene product. We conclude that the activity of the p R promoter is inhibited directly by ppGpp.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004380050674
Permalink