ISSN:
1432-203X
Keywords:
Key word: acid phosphatase – brefelding A –Nicotiana tabacum L. var. Xanthi – secretion – tobacco cells – tobacco protoplasts.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary. The effects of brefeldin A (BFA) on the secretion of acid phosphatase (APase) by tobacco protoplasts were investigated. Secretion of APase was inhibited by BFA in a dose-dependent manner, with a concomitant intracellular accumulation of the enzyme. The secreted APase was composed of two isoforms. BFA (10 μg/ml) inhibited the secretion of one of the isoforms without inhibiting that of the other, and this phenomenon explains the partial inhibition of APase secretion as a whole. The inhibition of APase secretion was accompanied by changes in the morphology of the Golgi apparatus and also by an increment in mass-density of cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050043
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