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  • 1
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    Capsaicin inhibits preferentially the NADH oxidase and growth of transformed cells in culture. (1995)
    National Academy of Sciences
    Details
    Publication Date: 1995-03-14
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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    PAPER CURRENT
  • 2
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    Intracellular membrane flow: reconstitution of transition vesicle formation and function in a cell-free system. (1987)
    National Academy of Sciences
    Details
    Publication Date: 1987-09-01
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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    PAPER CURRENT
  • 3
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    NADH oxidase activity (NOX) and enlargement of HeLa cells oscillate with two different temperature-compensated period lengths of 22 and 24 minutes corresponding to different NOX forms (2001)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: NOX proteins are cell surface-associated and growth-related hydroquinone (NADH) oxidases with protein disulfide-thiol interchange activity. A defining characteristic of NOX proteins is that the two enzymatic activities alternate to generate a regular period length of about 24 min. HeLa cells exhibit at least two forms of NOX. One is tumor-associated (tNOX) and is inhibited by putative quinone site inhibitors (e.g., capsaicin or the antitumor sulfonylurea, LY181984). Another is constitutive (CNOX) and refractory to inhibition. The periodic alternation of activities and drug sensitivity of the NADH oxidase activity observed with intact HeLa cells was retained in isolated plasma membranes and with the solubilized and partially purified enzyme. At least two activities were present. One had a period length of 24 min and the other had a period length of 22 min. The lengths of both the 22 and the 24 min periods were temperature compensated (approximately the same when measured at 17, 27 or 37 degrees C) whereas the rate of NADH oxidation approximately doubled with each 10 degrees C rise in temperature. The rate of increase in cell area of HeLa cells when measured by video-enhanced light microscopy also exhibited a complex period of oscillations reflective of both 22 and 24 min period lengths. The findings demonstrate the presence of a novel oscillating NOX activity at the surface of cancer cells with a period length of 22 min in addition to the constitutive NOX of non-cancer cells and tissues with a period length of 24 min.
    Keywords: Life Sciences (General)
    Type: Biochimica et biophysica acta (ISSN 0006-3002); Volume 1539; 3; 192-204
    Format: text
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    NASA TECHNICAL REPORTS
  • 4
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    Aqueous two-phase partition applied to the isolation of plasma membranes and Golgi apparatus from cultured mammalian cells (2000)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: Partitioning in dextran-poly(ethylene)glycol (PEG) aqueous-aqueous phase systems represents a mature technology with many applications to separations of cells and to the preparation of membranes from mammalian cells. Most applications to membrane isolation and purification have focused on plasma membranes, plasma membrane domains and separation of right side-out and inside-out plasma membrane vesicles. The method exploits a combination of membrane properties, including charge and hydrophobicity. Purification is based upon differential distributions of the constituents in a sample between the two principal compartments of the two phases (upper and lower) and at the interface. The order of affinity of animal cell membranes for the upper phase is: endoplasmic reticulum 〈mitochondria〈Golgi apparatus〈lysosomes and endosomes〈 plasma membranes. Salt concentrations and temperature affect partitioning behavior and must be precisely standardized. In some cases, it is more fortuitous to combine aqueous two-phase partition with other procedures to obtain a more highly purified preparation. A procedure is described for preparation of Golgi apparatus from transformed mammalian cells that combines aqueous two-phase partition and centrifugation. Also described is a periodic NADH oxidase, a new enzyme marker for right side-out plasma membrane vesicles not requiring detergent disruptions for measurement of activity.
    Keywords: Life Sciences (General)
    Type: Journal of chromatography. B, Biomedical sciences and applications (ISSN 1387-2273); Volume 743; 1-2; 377-87
    Format: text
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    NASA TECHNICAL REPORTS
  • 5
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    CHO cell enlargement oscillates with a temperature-compensated period of 24 min (2000)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: The rate of increase in cell area of CHO cells when measured at intervals of 1 min using a light microscope equipped with a video measurement system, oscillated with a minimum period of about 24 min. The pattern of oscillations paralleled those of the 24 min period observed with the oxidation of NADH by an external cell surface or plasma membrane NADH oxidase. The increase in cell area was non-linear. Intervals of rapid increase in area alternated with intervals of rapid decrease in area. The length of the 24 min period was temperature-compensated (approximately the same when measured at 14 degrees C, 24 degrees C or 34 degrees C) while the rate of cell enlargement increased with temperature over this same range of temperatures.
    Keywords: Life Sciences (General)
    Type: Biochimica et biophysica acta (ISSN 0006-3002); Volume 1498; 1; 44-51
    Format: text
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    NASA TECHNICAL REPORTS
  • 6
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    A light-responsive and periodic NADH oxidase activity of the cell surface of Tetrahymena and of human buffy coat cells (2000)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: Oxidation of external NADH (NADH is an impermeant substrate) by cells of Tetrahymena pyriformis oscillated with a period of 24-26 min. The period length in darkness (25.6 min) appeared to be slightly longer than the period in light (approximately 24 min). When Tetrahymena were placed in darkness for 30-50 min and then returned to light, a new maximum in the rate of NADH oxidation was observed 36-38 min (13 + 24) min after the beginning of the light treatment. The cell-surface NADH oxidase of human buffy coats (a mixture of white cells and platelets) also was periodic and light responsive.
    Keywords: Life Sciences (General)
    Type: Antioxidants & redox signalling (ISSN 1523-0864); Volume 2; 2; 289-300
    Format: text
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    NASA TECHNICAL REPORTS
  • 7
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    Soybean cell enlargement oscillates with a temperature-compensated period length of ca. 24 min (2001)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: Rate of enlargement of epidermal cells from soybean, when measured at intervals of 1 min using a light microscope equipped with a video measurement system, oscillated with a period length of about 24 min. This oscillation parallels the 24-min periodicity observed for the oxidation of NADH by the external plasma membrane NADH oxidase. The increase in length was not only non-linear, but intervals of rapid increase in area alternated with intervals of rapid decrease in area. The length of the period was temperature compensated, and was approximately the same when measured at 14, 24 and 34 degrees C even though the rate of cell enlargement varied over this same range of temperatures. These observations represent the first demonstration of an oscillatory growth behavior correlated with a biochemical activity where the period length of both is independent of temperature (temperature compensated) as is the hallmark of clock-related biological phenomena.
    Keywords: Life Sciences (General)
    Type: In vitro cellular & developmental biology. Plant : journal of the Tissue Culture Association (ISSN 1054-5476); Volume 37; 1; 19-23
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    NASA TECHNICAL REPORTS
  • 8
    Electronic Resource
    Electronic Resource
    Inhibition of the NADH oxidase activity of plasma membranes isolated from xenografts and cell lines by the antitumor sulfonylurea, N-(4-methylphenylsulfonyl)-N′-(4-chlorophenyl)urea (LY 181984) correlates with drug susceptibility of growth (1995)
    Springer
    Protoplasma 184 (1995), S. 203-208 
    Details
    ISSN: 1615-6102
    Keywords: K-ras ; H-ras ; Rat kidney cells ; Antitumor diarylsul-fonylureas ; Human colon xenografts ; HeLa cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Inhibition of NADH oxidase activity of plasma membranes isolated from a series of human xenografts and cell lines by the antitumor sulfonylurea, N-(4-methylphenylsulfonyl)-N′-(4-chlorophenyl) urea (LY 181984), correlated with the ability of the sulfonylurea to inhibit cell growth. Growth of rat kidney cells either untransformed or transformed with Kirsten-ras (K-ras) were unaffected by the sulfonylurea. Similarly, the NADH oxidase activity of isolated plasma membranes from K-ras transformed cells was unaffected by LY 181984. In contrast, when transformed with Harvey-ras (H-ras), both growth and NADH oxidase activity were inhibited. With the inactive but structurally related LY 181985 (N-4-methylphenyl-sulfonyl)-N′-(phenyl)urea), neither growth nor plasma membrane NADH oxidase activity of either sulfonylurea-susceptible or -resistant tissues or cell lines was inhibited. Both sulfonylureas were inactive with rat liver plasma membranes but NADH oxidase activity of plasma membranes and growth with HeLa cells was inhibited by the active (LY 181984) but not by the inactive (LY 181985) sulfonylurea. The findings suggest a possible correlation between inhibition of plasma membrane NADH oxidase activity by the antitumor sulfonylureas and their oncolytic action.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276921
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  • 9
    Electronic Resource
    Electronic Resource
    A cell-free approach to the study of membrane trafficking in frozen rat brains potentially applicable to frozen autopsy material (1996)
    Springer
    Protoplasma 190 (1996), S. 99-106 
    Details
    ISSN: 1615-6102
    Keywords: Aging ; Alzheimer's disease ; Brain ; Cell-free system ; Membranes ; Membrane trafficking ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A cell-free transfer system was used to measure capacity of brain membranes to support membrane renewal. To study transfer in brain, radiolabeled donor microsome fractions were prepared using brain slices from rats or frozen human brain autopsy specimens. Acceptor fractions, prepared from fresh or frozen rat brain or frozen human brain autopsy specimens, were immobilized on nitrocellulose. The complete reconstituted transfer system contained ATP plus ATP-regenerating system (or NADH) as a source of energy and brain cytosol. Slices of frozen brain incorporated acetate into membrane lipids with approximately the same efficiency as fresh brains. This efficiency declined with storage at 4 °C but only slowly. Donor fractions labeled with acetate from frozen slices exhibited specific transfer (37 °C minus 4 °C) of labeled membrane lipids with efficiencies comparable to fresh. The acceptor fraction could be prepared either from fresh or frozen material. Transfer was on the average two-fold stimulated by ATP at 37 °C compared to no ATP. Transfer also was stimulated by NADH. Analysis of linear transfer rates between 0 and 30 min revealed no significant effect of delay time or of time of prolonged storage on transfer efficiency beyond an initial decline of ca. 25% observed within the first two weeks after freezing. A decline of transfer was obtained with brains as the animals aged.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281198
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  • 10
    Electronic Resource
    Electronic Resource
    Golgi apparatus respond to the antitumor sulfonylurea N-(4-methylphenylsulfonyl)-N′-(4-chlorophenyl)urea (LY 181984) in sulfonylurea-susceptible but not in resistant cell lines (1994)
    Springer
    Protoplasma 182 (1994), S. 81-95 
    Details
    ISSN: 1615-6102
    Keywords: Antitumor sulfonylurea ; Golgi apparatus ; Monensin ; Sulfonylurea ; Cultured cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cell lines susceptible or resistant to the active antitumor sulfonylurea [N-(4-methylphenylsulfonyl)-N′-(4-chlorophenyl)-urea] (LY 181984) were treated with 100 μM sulfonylurea for 1 or 3 h followed by monensin for 1 h. With cell lines where growth was inhibited by the active sulfonylurea, swollen Golgi apparatus cisternae following treatment were fewer and smaller than in untreated cells. Overall the volume of monensin-responsive trans cisternae was reduced by about 50% to 75% in cells lines where the antitumor sulfonylurea was growth inhibitory. The swelling response was unaffected by sulfonylurea in sulfonylurea-unresponsive cells. The antitumor-inactive sulfonylurea [N-(4-methylphenylsulfonyl)-N′-(phenyl)urea] (LY 181985) was without effect on cisternal swelling with both susceptible and resistant cell lines. The results suggest a response of the trans Golgi apparatus to the active antitumor sulfonylurea that resulted in reduced acidification of the trans Golgi apparatus cisternae. This response appears to be restricted to susceptible cell lines where growth was inhibited by the active antitumor sulfonylurea but not in resistant cell lines where growth was unaffected by the active antitumor sulfonylurea.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01403470
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