ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Pseudomonas fluorescens EPS62e has been selected in a screening procedure for its high efficacy controlling Erwinia amylovora infections in flowers, immature fruits and young pear plants. We developed two monitoring methods which allowed specific detection and quantification of EPS62e by combining classical microbiological techniques with molecular tools. RAPD and unspecific-PCR fingerprints were used to differentiate EPS62e from other P. fluorescens strains. Differential amplified fragments from EPS62e were sequence characterized as SCAR markers and two primer pairs were designed and selected for their specificity against EPS62e. A SCAR primer pair was evaluated and validated for the assessment of population dynamics of EPS62e on pear plants under greenhouse conditions using plating and most probable number assays coupled to PCR. Both techniques were useful in monitoring the biological control agent. The population level of EPS62e after treatment was 7 log CFU (g f.w.)−1, which in turn decreased progressively to 4–5 log CFU (g f.w.)−1 after 17 days and then remained stable until the end of the assay 11 days later. The limit of detection of both monitoring methods developed was around 3 log CFU (g f.w.)−1, thus, providing a reliable tool for the analysis of EPS62e in greenhouse or field trials, and the assessment of threshold population levels for efficient biocontrol of fire blight.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The application of the biocontrol agent Pantoea agglomerans EPS125 to unwounded fruits was practically ineffective for control of postharvest blue mould caused by Penicillium expansum when the treatment and subsequent wounding and pathogen inoculation were separated by periods of unfavourable conditions. This was due to a rapid decrease in viability of the alocthonous introduced biocontrol agent in the intact peel surface. A system for osmoadaptation of the biocontrol agent was developed by combining saline osmotic stress and osmolyte amendment to the growth medium. Osmoadapted cells accumulated trehalose and glycine betaine (GB) intracellularly and showed a higher tolerance to desiccation than non-osmoadapted cells. Osmoadaptation in NaCl plus GB during inoculum preparation increased considerably survival on the peel surface of apple fruits. This effect was significant under low relative humidity (RH) and fluctuating RH conditions, but was not significant at high RH. Osmoadaptation significantly improved blue mould control under conditions where the standard biological control treatments were ineffective. The rot diameter was significantly reduced in apple fruits which were treated with EPS125 and incubated for several days under low, high or fluctuating RH, followed by wounding and inoculation of P. expansum. Growth of EPS125 with NaCl, either with or without the addition of GB, was an effective osmoadaptation treatment for improving blue mould rot control. However, the addition of GB to the NaCl amended growth medium increased 4–5-fold growth rate and OD of the cultures. This is an advantage for mass production of P. agglomerans EPS125 in a NaCl amended growth medium.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 48 (2004), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Population levels of total and ampicillin-resistant culturable bacteria and the putative horizontal bla gene acquirement from Bt-corn were studied in commercial fields of transgenic corn in Spain during the years 2000–2003. Commercial fields consisting of conventional corn (Dracma) and Bt176 transgenic corn (Compa CB) were located in three climatic regions. The effect of corn type, plant material, field location, stage of sampling and year of study were studied on total and ampicillin resistant bacterial population levels, on median effective dose and on the slope of the dose–response curve to ampicillin. None of the parameters measured were significantly different (P〈0.01) between transgenic and non-transgenic cornfields under the diverse conditions studied. However, in population levels of ampicillin resistant bacteria, the minimum difference between sample means to be significant with a likelihood of 80% was 8.9%. Specific detection of putative bacteria harbouring bla TEM-1 ampicillin resistance genes acquired from Bt176 corn was performed with a method based on the extraction of DNA from the culturable bacterial fraction and with PCR. Primers for PCR were targeted to the bla gene and the corresponding flanking regions present in the pUC18 cloning vector or the Bt176 construct. The culturable bacterial fraction of 144 field samples (up to 864 analysis, including ampicillin enrichments) was analysed by PCR. The estimated total number of bacteria analysed was 108. The level of detection of a transfer event according to the sensitivity of the methods used was 10−6. Four samples of transgenic and five of non-transgenic corn gave positive signals. However, the amplification products did not correspond to the ones expected from Bt176 or pUC18. The limitations of the sampling design and of the methods used are discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 4
    ISSN: 1573-8469
    Keywords: fosetyl ; phosphonate ; ethephon ; phosphomycin ; median effective dose ; antibacterial activity ; Pseudomonas syringae ; pear
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Several phosphonate derivatives including theoomycetic antifungal agents phosphonate andtris-o-ethylphosphonate (fosetyl), theethylene-releasing compound 2-chloroethylphosphonate(ethephon), and the antibiotic2-epoxypropylphosphonate (phosphomycin) were evaluatedfor in vitro and in planta activityagainst Pseudomonas syringae pv. syringae.Inhibition of colony growth in CYE agar byphosphonate, fosetyl and etephon was very slight(minimal inhibitory concentrations MIC= 0.31–;0.62 gHP $$O_3^{2 - } $$ /l). Also, survival of P. syringae pv. syringae in aqueous solutions ofphosphonate or fosetyl was high. Only phosphomycinshowed significant antibacterial activity invitro (MIC=10-20 µg HP $$O_3^{2 - } $$ /ml) comparedto streptomycin (1-2 µg a.i./ml). Potted pearplants irrigated with these chemicals and inoculatedwith Pseudomonas syringae pv. syringae had significantly less disease than non-treatedcontrols ( P〈0.001). Phosphomycin was the mostactive compound with a median effective dose(ED50) of less than 0.62 g HP $$O_3^{2 - } $$ /l.Activities of the other phosphonates were weak butconsistent between experiments. The ED50s on wholeplants were 2.1, 3.3, and 6.9 g HP $$O_3^{2 - } $$ /l for ethephon, phosphonate and fosetyl, respectively. TheED50of P. syringae pv. syringaeincreased from 6.5 in non-treated controls to 7.7-8.8log10 cfu/ml on plants treated with phosphonatesat 1.86 g HP $$O_3^{2 - } $$ /l. It was concluded thatdrench treatment with fosetyl is not a practicaloption for control of P. syringae pv. syringae on pear.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 5
    Publication Date: 2019
    Description: Convective hydrothermal systems have been extensively studied using electrical and electromagnetic methods given the strong correlation between low conductivity anomalies associated with hydrothermal brines and high temperature areas. However, studies addressing the application of similar geophysical methods to hot dry rock geothermal systems are very limited in the literature. The Timanfaya volcanic area, located on Lanzarote Island (Canary Islands), comprises one of these hot dry rock systems, where ground temperatures ranging from 250 to 605 °C have been recorded in pyroclastic deposits at shallow (〈70 m) depths. With the aim of characterizing the geophysical signature of the high ground temperature areas, three different geophysical techniques (ground penetrating radar, electromagnetic induction and magnetic prospecting) were applied in a well-known geothermal area located inside Timanfaya National Park. The area with the highest ground temperatures was correlated with the location that exhibited strong ground penetrating radar reflections, high resistivity values and low magnetic anomalies. Moreover, the high ground temperature imaging results depicted a shallow, bowl-shaped body that narrowed and deepened vertically to a depth greater than 45 m. The ground penetrating radar survey was repeated three years later and exhibited subtle variations of the signal reflection patterns, or signatures, suggesting a certain temporal variation of the ground temperature. By identifying similar areas with the same geophysical signature, up to four additional geothermal areas were revealed. We conclude that the combined use of ground penetrating radar, electromagnetic induction and magnetic methods constitutes a valuable tool to locate and study both the geometry at depth and seasonal variability of geothermal areas associated with hot dry rock systems.
    Electronic ISSN: 2072-4292
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Published by MDPI
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 6
    Publication Date: 2012-09-05
    Description: Background: The Biocontrol Peptide BP100 is a synthetic and strongly cationic a-helical undecapeptide with high, specific antibacterial activity against economically important plant-pathogenic bacteria, and very low toxicity. It was selected from a library of synthetic peptides, along with other peptides with activities against relevant bacterial and fungal species. Expression of the BP100 series of peptides in plants is of major interest to establish disease-resistant plants and facilitate molecular farming. Specific challenges were the small length, peptide degradation by plant proteases and toxicity to the host plant. Here we approached the expression of the BP100 peptide series in plants using BP100 as a proof-of-concept. Results: Our design considered up to three tandemly arranged BP100 units and peptide accumulation in the endoplasmic reticulum (ER), analyzing five BP100 derivatives. The ER retention sequence did not reduce the antimicrobial activity of chemically synthesized BP100 derivatives, making this strategy possible. Transformation with sequences encoding BP100 derivatives (bp100der) was over ten-fold less efficient than that of the hygromycin phosphotransferase (hptII) transgene. The BP100 direct tandems did not show higher antimicrobial activity than BP100, and genetically modified (GM) plants constitutively expressing them were not viable. In contrast, inverted repeats of BP100, whether or not elongated with a portion of a natural antimicrobial peptide (AMP), had higher antimicrobial activity, and fertile GM rice lines constitutively expressing bp100der were produced. These GM lines had increased resistance to the pathogens Dickeya chrysanthemi and Fusarium verticillioides, and tolerance to oxidative stress, with agronomic performance comparable to untransformed lines. Conclusions: Constitutive expression of transgenes encoding short cationic a-helical synthetic peptides can have a strong negative impact on rice fitness. However, GM plants expressing, for example, BP100 based on inverted repeats, have adequate agronomic performance and resistant phenotypes as a result of a complex equilibrium between bp100der toxicity to plant cells, antimicrobial activity and transgene-derived plant stress response. It is likely that these results can be extended to other peptides with similar characteristics.
    Electronic ISSN: 1471-2229
    Topics: Biology
    Published by BioMed Central
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 7
    Publication Date: 2014-04-23
    Description: Background: Cecropin A is a natural antimicrobial peptide that exhibits rapid, potent and long-lasting lytic activity against a broad spectrum of pathogens, thus having great biotechnological potential. Here, we report a system for producing bioactive cecropin A in rice seeds. Results: Transgenic rice plants expressing a codon-optimized synthetic cecropin A gene drived by an endosperm-specific promoter, either the glutelin B1 or glutelin B4 promoter, were generated. The signal peptide sequence from either the glutelin B1 or the glutelin B4 were N-terminally fused to the coding sequence of the cecropin A. We also studied whether the presence of the KDEL endoplasmic reticulum retention signal at the C-terminal has an effect on cecropin A subcellular localization and accumulation. The transgenic rice plants showed stable transgene integration and inheritance. We show that cecropin A accumulates in protein storage bodies in the rice endosperm, particularly in type II protein bodies, supporting that the glutelin N-terminal signal peptides play a crucial role in directing the cecropin A to this organelle, independently of being tagged with the KDEL endoplasmic reticulum retention signal. The production of cecropin A in transgenic rice seeds did not affect seed viability or seedling growth. Furthermore, transgenic cecropin A seeds exhibited resistance to infection by fungal and bacterial pathogens (Fusarium verticillioides and Dickeya dadantii, respectively) indicating that the in planta-produced cecropin A is biologically active. Conclusions: Rice seeds can sustain bioactive cecropin A production and accumulation in protein bodies. The system might benefit the production of this antimicrobial agent for subsequent applications in crop protection and food preservation.
    Electronic ISSN: 1471-2229
    Topics: Biology
    Published by BioMed Central
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 8
    Publication Date: 2018-03-06
    Description: Plant elicitor peptides (Peps) are endogenous molecules that induce and amplify the first line of inducible plant defense, known as pattern-triggered immunity, contributing to protect plants against attack by ...
    Electronic ISSN: 1471-2156
    Topics: Biology
    Published by BioMed Central
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 9
    Publication Date: 2012-12-03
    Description: Carbohydrates are considered as promising templates for the display of multiple copies of antimicrobial peptides. Herein, we describe the design and synthesis of chimeric structures containing two or four copies of the antimicrobial peptides KKLFKKILKYL-NH2 (BP100) and KKLfKKILKYL-NH2 (BP143) attached to the carbohydrate template cyclodithioerythritol (cDTE) or α-D-galactopyranoside (Galp). The synthesis involved the preparation of the corresponding peptide aldehyde followed by coupling to an aminooxy-functionalized carbohydrate template. After purification, the multivalent display systems were obtained in high purities (90–98%) and in good yields (42–64%). These compounds were tested against plant and human pathogenic bacteria and screened for their cytotoxicity on eukaryotic cells. They showed lower MIC values than the parent peptides against the bacteria analyzed. In particular, the carbopeptides derived from cDTE and Galp, which contained two or four copies of BP100, respectively, were 2- to 8-fold more active than the monomeric peptide against the phytopathogenic bacteria. These results suggest that preassembling antimicrobial peptides to multimeric structures is not always associated with a significant improvement of the activity. In contrast, the carbopeptides synthesized were active against human red blood cells pointing out that peptide preassembly is critical for the hemolytic activity. Notably, peptide preassembly resulted in an enhanced bactericidal effect.
    Print ISSN: 2195-951X
    Electronic ISSN: 1860-5397
    Topics: Chemistry and Pharmacology
    Published by Beilstein-Institut
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 10
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...