ISSN:
1572-9931
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Temperature-sensitive (ts) mutants were isolated in a cell line ofDrosophila melanogaster, GM1, by ethyl methanesulfate treatment. Two of them,ts15 andts58, formed colonies at 23°C but not at 30° when inoculated at densities of/or less than 105 cells per 60×15-mm dish. By using thesets mutants, cell fusion was attempted with polyethylene glycol (PEG) 6000. Several colonies per dish developed at 30°C when differentts mutants were mixed, treated with PEG, and inoculated at a density of 104 cells per dish. Cells in some of the colonies thus developed were propagated and their temperature-sensitive character and karyotypes were studied. The results indicated that cell fusion could be induced with PEG and that the cells which formed colonies at 30°C after PEG treatment were the hybrids in which the temperature-sensitive lesions in the mutants were complemented.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01538979
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